A NEW CONCEPT FOR THE SEPARATION OF METAL CHELATES BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

制备了2-（4-吡啶基）乙基键合硅胶（4-PES）柱。在<II><II><II><II>预先制备的4，5-二氢咪唑基键合硅胶的4-PES柱上分离了Zn^ -、Co^ -、Ni^ -和Cu^ -四苯基卟啉（TPP）配合物（4，5-DHIPS）和2-（2-吡啶基）乙基键合硅胶（2-PES）柱以及正相模式的商业氨丙基键合硅胶（APS）柱中，金属-TPP络合物的保留最强，也就是说，在<II><II>4-PES柱上观察到Zn^ -和Co^ -TPPs。金属-TPPs在这些柱中的分离机理可能主要是基于络合物中的中心金属与固定相中的固定化中性配体之间的配位作用。

Newly prepared was a 2- (4-pyridyl) ethyl bonded silica gel (4-PES) column for high-perfomance liquid chromatography. Zn^<II>-, Co^<II>-, Ni^<II>-, and Cu^<II>-tetraphenylporphine (TPP) complexes were separated on this 4-PES column, previously prepared 4,5-dihydroimidazolyl bonded silica gel (4,5-DHIPS) and 2- (2-pyridyl) ethyl bonded silica gel (2-PES) columns as well as a commercial aminopropyl bonded silica gel (APS) column with normal phase mode.Among them, the strongest retention of the metal-TPP complexes which are considered to be coordinatively unsaturated, i.e., Zn^<II>-and Co^<II>-TPPs, was observed on the 4-PES column. The separation mechanism of the metal-TPPs in these columns may mainly be based on the coordination interaction between the central metal in the complex and the immobilized neutral ligand in the stationary phase.