Molecular mechanism of salt tolerance in yeast

酵母耐盐的分子机制

• 批准号: 07456050
• 负责人: MIYAKAWA Tokichi
• 金额: $ 5.25万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07456050/
• 关键词: veast; salt tolerance; calcineurin; cation homeostasis; Salt tolerance; 酵母; カルシニューリン; 塩耐性; Ca^<2+>; カチオンホメオスタシス

We investigated the mechanism for the maintenance of cation homeostasis in the yeast Saccharomyces cerevisiae. Followings are our findings.1.Salt tolerance mechanism that is regulated by calcineurin and protein kinase A :W have shown that the expression of ENA1 gene encoding a sodium extrusion pump ATPase which is responsible for decreasing salt concentrations under high salt environmentalconditions, is regulated positively by calcineurin and negatively by protein kinase A.2.Isolation and characterization of high-salt sensitive mutants :We isolated 1370 salt sensitive mutants, and classified the mutants to 30 complementation groups. Mutants that were suggested by genetic analysis to be defective in the functions that act downstream of calcineurin were selected (5 complementation groups). Genes that complement the defect of the mutant were isolated. Amongs the genes identified, a gene that encode putative transcription factor was found. The function of this gene in salt tolerance is under investigation.3.Isolation of muticopy suppressor genes that complement the defect of calcineurin-deficient mutant :Two each of the genes that encode putative transcription factor and putative membrane transporters were obtained by the screening. The function of these genes is under investigation by gene disruption experiment.

我们研究了酿酒酵母中维持阳离子稳态的机制。我们的发现如下。1.钙调神经磷酸酶和蛋白激酶A调节的耐盐机制：W已经表明，编码钠挤压泵ATP酶的ENA1基因的表达负责在高盐环境条件下降低盐浓度，受钙调神经磷酸酶正向调节，蛋白激酶A负向调节。2.高盐敏感突变体的分离和表征：我们 分离出 1370 个盐敏感突变体，并将突变体分为 30 个互补组。选择通过遗传分析表明作用于钙调神经磷酸酶下游的功能有缺陷的突变体（5个互补组）。分离出补充突变体缺陷的基因。在鉴定的基因中，发现了编码假定转录因子的基因。该基因的耐盐功能正在研究中。3.补充钙调磷酸酶缺陷突变体缺陷的多拷贝抑制基因的分离：筛选得到编码假定的转录因子和假定的膜转运蛋白的基因各2个。这些基因的功能正在通过基因破坏实验进行研究。

项目成果

K. Miyahara: "yAP1 ad yAP2 mediaed, heat shock-inducible trauscriptlonal activation of ABC gcues." Curr. Genet. 29. 103-105 (1996)

K. Miyahara：“yAP1 和 yAP2 介导的 ABC gcues 的热休克诱导转录激活。”

平田大: "細胞周期制御の分子機構 分担執筆" 共立出版, 8 (1996)

Dai Hirata：“细胞周期控制的分子机制 - 合著者”Kyoritsu Shuppan，8 (1996)

T.Nakamura: "Genetic evidence for the functional redundancy of the calcineurin-and Mpk1-mediated pathways in the regulation of cellular ecents important for growth in yeast" Mol.Gen.Genet.251. 211-219 (1996)

T.Nakamura：“钙调神经磷酸酶和 Mpk1 介导的途径在调节对酵母生长很重要的细胞事件中存在功能冗余的遗传证据”Mol.Gen.Genet.251。

K.Miyahara: "The involvement of the yeast multidrugresistance protein Pdrs and Sng2 in cation resistance" FEBS Letters. 399・3. 317-320 (1996)

K.Miyahara：“酵母多重耐药蛋白 Pdrs 和 Sng2 在阳离子耐药性中的参与”FEBS Letters 399·3（1996）。

P.Cliften: "SYR2,a gene necessary for syrirgonuycin" Microbiology. 142. 477-484 (1996)

P.Cliften：“SYR2，丁香菌素必需的基因”微生物学。