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Experimental studies of small bowel transplantation using newborn intestines.

利用新生儿肠道进行小肠移植的实验研究。

基本信息

批准号：
07457279
负责人：
MIYATA Michio.MD.PhD.
金额：
$ 3.78万
依托单位：
Jichi Medical School
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457279/
关键词：
small bowel transplantation newborn rejection GVHD neovasculization 血管新生新生仔ドナー GVHD 消化管運動

项目摘要

Using the characters that the newborn organs are immunologically immatured, we studies exprimental small bowel transplantation (SBT) using newborn intestines in rats.In a singeneic combination, newborn grafts (intestine, spleen, liver, kidney, heart and pancreas) were transplanted into the subcutaneous space. Intestinal grafts obtained from the different-time newborns were also tested from the point of neovasculization rate. To show the absorbable ability of newborn intesting, the recipients were totally reconstructed by use of the newborn intestine grafted at the greater omentum. The grafts were assessed morphologically, histologically, electrophysiologically, and nutritionally. The rate of neovasculization in spleen, intestine, kidney, and heart was 87.5,70.6,41.7 and 20.0%, respectively. However, no neovasculization was observed in liver nor in pancreas. The ability of revasculization was rapidly lost after birth, and non using the newborn surviving longer than 11 days. The intestinal graft transplanted at the greater omentum showed normal EEG wave and could be reconstructed, resulting in the growth of the recipient.In an allogeneic combination of LEW-into-PVG/c, a short course of 15-Deoxyspergualin prolonged N-SBT.Furthermore, splenectomy and/or thrmectomy on the recipients were efficient on graft survival. In a xenogeneic combination of B6-into-LEW,cyclophosphamide plus tacrolimus were effective on the graft survival, but histologically impaired. However, additional treatment of hepatic lymphomyeloid cell infusion could improved histologically.On the contrary, SBT using the matured intestine showed sever rejection reaction in the acute and chronic phase. A new imunosuppressant, FTY720 was effective on combination therapy with conventional immunosuppressive drugs.

利用新生器官免疫不成熟的特点，我们研究了新生大鼠小肠移植(SBT)，将新生小肠移植到皮下腔内。从新生血管的角度对不同时间的新生儿移植肠片进行了检测。为了显示新生儿肠管的吸收能力，受体采用新生儿大网膜移植的方法进行完全重建。对移植物进行了形态、组织学、电生理学和营养方面的评估。脾、肠、肾和心脏的新生血管发生率分别为87.5%、70.6%、41.7%和20.0%。然而，在肝脏和胰腺中都没有观察到新生血管。新生血管重建能力在出生后迅速丧失，未使用的新生儿存活超过11天。同种异体Lew-into-PVG/c联合15-脱氧精灵可延长N-SBT。此外，对受体进行脾切除和/或去栓术可有效提高移植物的存活率。在B6-Into-LEW的异种组合中，环磷酰胺和他克莫司对移植物的存活有效，但组织学上受到损害。然而，补充肝淋巴样细胞输注可以改善组织学，相反，使用成熟肠段的SBT在急性期和慢性期表现出严重的排斥反应。FTY720是一种新型免疫抑制剂，与常规免疫抑制剂联合治疗效果显著。