Prenatal DNA diagnosis by maternal blood

母血产前DNA诊断

• 批准号: 07457610
• 负责人: YANAIHARA Takumi
• 金额: $ 0.45万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07457610/
• 关键词: prenatal diagnosis; maternal blood; fetal DNA diagnosis; 有核赤血球; 出生前遺伝子診断

Recently it has known that nucleated fetal cells are found in maternal blood. Until now, prenatal diagnosis from fetal cells is performed invasively. An attempt was made to develop prenatal DNA diagnosis using fetal nucleated erythrocytes (NRBCs) from maternal blood. NRBCs from maternal blood (8-13 weeks of gestation) were obtained by percoll using discontinous density gradient method and cells were collected indivisually by micromanipulator. Genomic DNA was amplified by PEP-PCR method. We have established the identification method whether NRBCs are derived from fetus or not by HLA-DQalpha typing. This method will be applicable for female fetuses. We have published prenatal diagnosis for Duchenne muscular dystrophy, ornitine decarbamylase deficiency and fetal Rh blood type using this method. Furthermore, this method of prenatal diagnosis can be applied to other genomic disorders and is expected to become preffered method of noninvasive prenatal diagnosis of DNA.

最近，人们知道在母体血液中发现了有核胎儿细胞。到目前为止，利用胎儿细胞进行产前诊断是有侵入性的。利用孕妇外周血中的胎儿有核红细胞(NRBCs)进行产前DNA诊断。采用Percoll不连续密度梯度法从母血(孕8~13周)中分离NRBC，用显微操作仪进行个体化采集。用PEP-PCR方法扩增基因组DNA。我们建立了用人类白细胞抗原DQAlpha分型鉴定NRBCs是否来源于胎儿的方法。这种方法将适用于女性胎儿。我们用这种方法发表了Duchenne肌营养不良症、鸟氨酸脱氨酶缺乏症和胎儿Rh血型的产前诊断。此外，这种产前诊断方法还可以应用于其他基因组疾病，有望成为DNA无创性产前诊断的首选方法。

项目成果

矢内原巧他: "妊婦末梢血中有核赤血球を用いた胎児出生前診断法" 産婦人科の治療. 72. 106-111 (1996)

Takumi Yanaihara 等人：“利用孕妇外周血中有核红细胞对胎儿进行产前诊断”《妇产科》72. 106-111 (1996)。

Akihiko Sekizawa, Akira Watanabe, Takehiko Kimura, Takeshi Sato, Hiroshi Saito, Takumi Yanaihara: "Prenatal diagnosis of the RhD blood type using a single nucleated erythrocyte in maternal blood." Obstetrics and Gynecology. 87. 501-501 (1996)

Akihiko Sekizawa、Akira Watanabe、Takehiko Kimura、Takeshi Sato、Hiroshi Saito、Takumi Yanaihara：“使用母血中的单核红细胞对 RhD 血型进行产前诊断。”

Akihiko Sekizawa, Takehiko Kimura, Takashi Matsuoka, Hiroyuki Kurihara, Akira Watanabe, Hiroshi Saito, Takumi Yanaihara, Takeshi Sato: "Prenatal diagnosis using fetal nucleated erythrocytes from maternal blood." Obstetrical and Gynecolo. 72(in Japan). 106

Akihiko Sekizawa、Takehiko Kimura、Takashi Matsuoka、Hiroyuki Kurihara、Akira Watanabe、Hiroshi Saito、Takumi Yanaihara、Takeshi Sato：“使用来自母体血液的胎儿有核红细胞进行产前诊断。”

Akihiko Sekizawa, Atsushi Taguchi, Akira Watanabe, Takehiko Kimura, Hiroshi saito, Takumi Yanaihara, Takeshi Sato: "Analysis of HLA-DQalpha sequences for prenatal diagnosis in single fetal cells from maternal blood." Human Genetics. (In Prss). (1998)

Akihiko Sekizawa、Atsushi Taguchi、Akira Watanabe、Takehiko Kimura、Hiroshi Saito、Takumi Yanaihara、Takeshi Sato：“用于母血单个胎儿细胞产前诊断的 HLA-DQalpha 序列分析。”

Akira Watanabe, Akihiko Sekizawa, Takehiko Kimura, Takeshi Sato, Hiroshi Saito, Takumi Yanaihara: "Noninvasive prenatal diagnosis of the RhD blood type using a single nucleated erythrocyte in maternal biood." Journal of Japanese O Nuturition and Metabo.2.

Akira Watanabe、Akihiko Sekizawa、Takehiko Kimura、Takeshi Sato、Hiroshi Saito、Takumi Yanaihara：“使用母体血液中的单核红细胞对 RhD 血型进行无创产前诊断。”