Precise Heat Capacity Measurement of Dilute Protein Aqueous Solutions by AC-Calorimetry

为了建立测定天然蛋白质和变性蛋白质在稀水溶液中的热容并测定其热力学稳定性的方法，设计并制作了一种交流量热计。对氯化钾、尿素、葡萄糖、麦芽糖和蛋白质溶菌酶的水溶液进行了测试。虽然发现与热容相对应的仪器信号与溶质浓度成正比，但其绝对值包含相当大的误差，导致再现性差。结果表明，该设计不适用于实际测量蛋白质在溶液中原形的热容，并讨论了改进方法的几点。

In order to establish a method to heat capacity of native and denatured proteins in aqueous dilute solution and to determine their thermodynamic stability, an AC-calorimeter was designed and constructed. Testing was performed on the aqueous solutions of potassium chloride, urea, glucose, maltose and protein lysozyme.Although the instrumental signal corresponding to the heat capacity was found to be proportional to the solute concentrations, its absolute magnitude contained fairly large errors resulting in the poor reproducibility. It was concluded that the design is not applicable to the practical measurement of the heat capacity of proteins in solution in its original form and some possible points to improve the method were discussed.