Intracellular responses by stimulation of receptors in neurons and related cells
通过刺激神经元和相关细胞中的受体而产生细胞内反应
基本信息
- 批准号:07458205
- 负责人:
- 金额:$ 5.25万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (B)
- 财政年份:1995
- 资助国家:日本
- 起止时间:1995 至 1996
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Neurons and related cells in the brain receive diverse stimuli for receptors in the plasma membranes, which include nerve impulses, neurotransmitters, neuropeptides, autacoids, hormones, cytokines, and growth factors. Responses of the cells include various types such as the production of second messengers and activation of tyrosine kinases. The intracellular calcium ion (Ca^<2+>) is established to be one of second messengers and its concentration in the cells is strictly regulated. The purposes of the present study are to elucidate the intracellular responses elicited by the increase in Ca^<2+> on the basis of stimulation of receptors in the plasma membranes. We used primarily cultured neurons of the hippocampus, astrocytes from cerebrum and cultured granule cells from cerebellum, established cell lines such as PC12 cells. NG108-15 cells, C6 glioma cells, fibroblast 3Yl cells and brain slices. In an attempt to elucidate the functions of the central nervous system through Ca^<2+> signaling pathways, we examined the dynamic state of activation of Ca^<2+>/calmodulin-dependent protein kinase II (CaM kinase II) in the cell systems. We found that CaM kinase II is activated through elevation of intracellular Ca^<2+> in response to the extracellular stimuli and is involved in the cellular functions of the central nervous system, such as neuronal differentiation of PC12 cells, gene expression in astrocytes and long-term potentiation in the CAl area of the hippocampus. Ca^<2+> is also involved in the activation of mitogenactivated protein kinase in neurons and astrocytes. Furthermore, with concomitant activation of the enzyme, phosphorylation of endogenous substrates was studied.
脑内神经元及相关细胞接受质膜受体的多种刺激,包括神经冲动、神经递质、神经肽、类自身激素、激素、细胞因子、生长因子等。细胞的反应包括多种类型,如第二信使的产生和酪氨酸激酶的激活。细胞内钙离子(Ca^<2+>)被确定为第二信使之一,其在细胞内的浓度受到严格调控。本研究的目的是在刺激质膜受体的基础上阐明Ca^<2+>增加所引起的细胞内反应。我们主要使用培养的海马神经元、大脑星形胶质细胞和培养的小脑颗粒细胞,建立了PC12细胞等细胞系。NG108-15细胞、C6胶质瘤细胞、成纤维细胞3Yl细胞和脑切片。为了阐明通过Ca^<2+>信号通路中枢神经系统的功能,我们检测了细胞系统中Ca^<2+>/钙调素依赖性蛋白激酶II (calmodulin-dependent protein kinase II, CaM kinase II)的激活动态。我们发现,在细胞外刺激下,CaM激酶II通过细胞内Ca^<2+>的升高而被激活,并参与中枢神经系统的细胞功能,如PC12细胞的神经元分化、星形胶质细胞的基因表达和海马CAl区的长期增强。Ca^<2+>也参与神经元和星形胶质细胞中有丝分裂原激活蛋白激酶的激活。此外,伴随酶的激活,内源性底物的磷酸化被研究。
项目成果
期刊论文数量(67)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
X.Sun, T.Tashiro, S.Hirai, H.Yamamoto, E.Miyamoto and Y.Komiya: "Preparation of tau from the peripheral nerve : Presence of insoluble low molecular weight tau with high phosphorylation." Biochem.Biophys.Res.Commun.210. 338-344 (1995)
X.Sun、T.Tashiro、S.Hirai、H.Yamamoto、E.Miyamoto 和 Y.Komiya:“从周围神经制备 tau 蛋白:存在高度磷酸化的不溶性低分子量 tau 蛋白。”
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H.Yamamoto, F.Arakane, T.Ono, K.Tashima, E.Okumura, K.Yamada, S.Hisanaga, K.Fukunaga, T.Kishimoto and E.Miyamoto: "Phosphorylation of myristoylated alanine-rich C kinase substrate (MARCKS) by proline-directed protein kinases and its dephosphorylation." J.
H.Yamamoto、F.Arakane、T.Ono、K.Tashima、E.Okumura、K.Yamada、S.Hisanaga、K.Fukunaga、T.Kishimoto 和 E.Miyamoto:“富含肉豆蔻酰化丙氨酸的 C 激酶底物的磷酸化
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S.Hokimoto, H.Yasue, K.Fujimoto, R.Sakata and E.Miyamoto: "Increased angiotensin converting enzyme activity in left ventricular aneurysm of patients after myocardial infarction." Cardiovascular Research. 29. 664-669 (1995)
S.Hokimoto、H.Yasue、K.Fujimoto、R.Sakata 和 E.Miyamoto:“心肌梗塞后左心室动脉瘤患者血管紧张素转换酶活性增加。”
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N.Yanagihara, Y.Oishi, H.Yamamoto, M.Tsutsui, J.Kondoh, T.Sugiura, E.Miyamoto and F.Izumi: "Phosphorylation of chromogranin A and catecholamine secretion stimulated by elevation of intracellular Ca^<2+> in cultured bovine adrenal medullary cells." J.Biol.
N.Yanagihara、Y.Oishi、H.Yamamoto、M.Tsutsui、J.Kondoh、T.Sugiura、E.Miyamoto 和 F.Izumi:“细胞内 Ca^2 升高刺激嗜铬素 A 的磷酸化和儿茶酚胺分泌
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N.Yanagihara: "Phosphorylation of chromogranin A and catecholamine secretion stimulated by elevation of intracellular Ca^<2+> in cultured bovine adrenal medullary cells" J.Biol.Chem.271. 17463-17468 (1996)
N.Yanagihara:“培养的牛肾上腺髓质细胞中细胞内Ca 2+ 的升高刺激嗜铬素A的磷酸化和儿茶酚胺分泌”J.Biol.Chem.271。
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MIYAMOTO Eishichi其他文献
MIYAMOTO Eishichi的其他文献
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{{ truncateString('MIYAMOTO Eishichi', 18)}}的其他基金
Establishment of cell models on transfection of functional protein and the study on brain signal transduction
功能蛋白转染细胞模型的建立及脑信号转导研究
- 批准号:
12557011 - 财政年份:2000
- 资助金额:
$ 5.25万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular and cytobiological study on regulation of synapse
突触调控的分子和细胞生物学研究
- 批准号:
11694295 - 财政年份:1999
- 资助金额:
$ 5.25万 - 项目类别:
Grant-in-Aid for Scientific Research (B).
Molecular cytobiological study on hippocampal LTP and LTD
海马LTP和LTD的分子细胞生物学研究
- 批准号:
09044324 - 财政年份:1997
- 资助金额:
$ 5.25万 - 项目类别:
Grant-in-Aid for international Scientific Research
Molecular cytobiological study on CaィイD12+ィエD1 signaling in the cells with cultured cells
培养细胞中CaiD12+CaiD1信号传导的分子细胞生物学研究
- 批准号:
09480223 - 财政年份:1997
- 资助金额:
$ 5.25万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Study on the mechanism of brain plasticity
脑可塑性机制研究
- 批准号:
07044281 - 财政年份:1995
- 资助金额:
$ 5.25万 - 项目类别:
Grant-in-Aid for international Scientific Research
Establishment of stable cells by induction of cDNAs of functional proteins and preparation of models for evaluation of drug efficacy for creation of new drugs
通过功能蛋白cDNA诱导建立稳定细胞并制备用于新药研发的药效评价模型
- 批准号:
07557195 - 财政年份:1995
- 资助金额:
$ 5.25万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Molecular and Cellular Biological Study on the Actions of Intracellular Calcium Ion in Cultured Cells
培养细胞内钙离子作用的分子和细胞生物学研究
- 批准号:
05454668 - 财政年份:1993
- 资助金额:
$ 5.25万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Study on long-term potentiation of brain hippocampus
大脑海马长时程增强研究
- 批准号:
04044134 - 财政年份:1992
- 资助金额:
$ 5.25万 - 项目类别:
Grant-in-Aid for international Scientific Research
The Cytophamacological Study on the Responses of the Receptors in the Cell System
细胞系统受体反应的细胞病理学研究
- 批准号:
02454139 - 财政年份:1990
- 资助金额:
$ 5.25万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
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- 批准号:
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Innate Intracellular Response to West Nile Virus
对西尼罗河病毒的先天细胞内反应
- 批准号:
6857794 - 财政年份:2006
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