Construction of the Systems with Memory of Molecules and Thir Application to Artificial antibody Catalysts

在这项研究中，我们尝试开发一种新的分子印迹方法，使可溶性宿主具有客体分子大小和形状的记忆。-环糊精（- cd），作为。水溶液中的宿主分子已被用作实现这一目标的关键化合物。首先，在1-萘乙酸（模板客体）和b-CD的混合物中加入氯化戊二酸、氯化丙二酸和氯化琥珀酸。在所含客体分子周围发生的分子内交联可以形成具有客体大小和形状记忆的空腔。反应结束后，通过过滤去除不溶物，并对溶液进行脱盐。将溶液加入丙酮中，收集沉淀。加入具有记忆能力的改性CD后，荧光强度的变化通过结合常数（1:1的化学计量）来评价其记忆能力的程度。客体诱导的荧光强度变化可以通过1:1的主客体化学计量来分析。用相对结合常数评价客体选择性，以萘为标准。当以1 -萘乙酸为模板客体时，l-甲氧基萘与改性CD的结合强度是与j3-CD本身的两倍。另一方面，当2-萘烯乙酸为模板客体时，2-甲氧基萘和2-乙氧基萘与改性cd的结合强度为2倍。作为另一种尝试，将模板客体结合到柱凝胶和含有7个氨基或乙二胺单元的cd进行柱层析。在这种情况下，Scuff的碱形成是交联反应。该方法已被证明是一种很有前途的新型分子印迹方法。

In this study, attempts have been made to develop a new molecular imprinting method that gives soluble hosts with a memory of size and shape of guest molecules. beta-CycIodextrin (beta-CD), which acts as .a host molecule in aqueous solution, has been used as a key compound to reach the aim. First, to a mixture of 1-naphthaleneacetic acid (template guest) and b-CD were added glutalalic acid chloride, malonyl acid chloride, and succinic acid chloride. The intramolecular crosslinking occurring around the included guest molecule may form the cavity which has a memory of the-size and shape of the guest. After the reaction, insoluble matter was removed by filtration-and the solution was desalted. The solution was added to acetone, and the precipitates were collected. The extents in memory ability were evaluated by obtaing binding constants (1 : 1 stoichiometry) from the changes in the fluorescence intensity induced by addition of the modified CD with memory ability. The guest induced variations in the fluorescence intensity could be analized by 1 : 1 host-guest stoichiometry. The guest selectivities were evaluated by the relative binding vonstants, making 100 for naphthalene as a standard. When I-naphthaleneacetic acid was used as the template guest, l-methoxynaphthalene was bound two-times stronger to the modified CD than to j3-CD itself. On the other hand, when 2-naphihaleneacetic acid was a template guest, 2-methoxynaplithalene and 2-ethoxynaphthalene were bound two times stronger to the modified CD.As another attempt, column chromatography was performed by using template guest bound to column gel and CDs with seven amino or ethylendiamine units. In this case, Scuff s base formation is the crosslinking reaction. This method has been proven to be a promissing method as new molecular imprinting method.