Respiratory chain of Gram-positive bacteria for better energy yield

对常用细菌的能量产生量及其创新进行了研究。嗜热菌的呼吸链由NADH脱氢酶-MK-7-b6c1复合体-CaA3氧化酶-O_2组成。我们还发现细胞色素bd型喹酚氧化酶和细胞色素b(o/a)3型细胞色素c-551氧化酶正在发挥作用。在中嗜性杆菌中，细胞色素Aa3型喹酚氧化酶是主要的末端氧化酶，这就是这些细菌H^/O比低的原因。为了比较这些血红素-铜氧化酶家族，我们研究了幽门螺杆菌细胞色素Cb型细胞色素氧化酶及其底物细胞色素c-553。以嗜热脂肪芽孢杆菌及其缺失CaA3型细胞色素C氧化酶、含有BD型喹酚氧化酶的突变株为主要末端氧化酶，研究了作为H泵呼吸复合体结合体的细胞H^/O比与细菌生长产量的直接相关性。对氨基酸发酵专用的柯林型细菌的呼吸链进行了研究，并纯化了BD型喹酚氧化酶和Aa3型细胞色素氧化酶两种末端氧化酶。我们还成功地克隆了它们的结构基因。

The following results were obtained on the energy yield of usuful bacteria and its inovation.1. Respiratory chain of thermophilic bacilli was analyzed to be composed of NADH dehydrogenase- MK-7- b6c1 complex- caa3 oxidase- O_2. We also found cytochrome bd-type quinol oxidase and cytochrome b(o/a) 3-type cytochrome c-551 oxidase are working.2. In mesophilic bacilli cytochrome aa3-type quinol oxidase was the main terminal oxidase, and thus this is the reason for low H^+/O ratio around 4 of these bacteria.3. To compare these heme-Cu oxidase family, Helicobacter pylori cytochrome cb-type cytohrome oxidase and its substrate cytochrome c-553 were investigated.4. Using Bacillus stearothermophilus and its mutant which lacks caa3-type cytochrome c oxidase and contains bd-type quinol oxidase as the main terminal oxidase, direct corelationship between H^+/O ratio of the cell as the combination of H^+-pumping respiratory complexes and the growth yield of the bacterium.5. The respiratory chain of Coryne-form bacteria, especially used in amino acid fermentation was examined, and two terminal oxidase, bd-type quinol oxidase and aa3-type cytochrome oxidase were purified. We also succeeded in cloning their structure genes.