权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Production of functional malic acid polymer by Aureobasidium and its application

短梗霉生产功能性苹果酸聚合物及其应用

基本信息

批准号：
07556088
负责人：
NAKAHARA Tadaatsu
金额：
$ 1.66万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07556088/
关键词：
poly (malic acid)microbiol production biodegradable polymer

项目摘要

Poly (L-malic acid), PMA,production by the non-growing cells of Aureobasidium sp.strain A-91 was investigated. Reaction conditions were optimized using non-growing cells of strain A-91. The addition of CuSO_4 to the cell preparation medium was found to be effective to prepare active cells for PMA production. The non-growing cells of the strain yielded 80.0g/l PMA during 5 days' incubation at 30ﾟC under optimal conditions (160g/l glucose and 40g/l CaCO_3).On the other hand, strain A-91 also produces pullulan and polyol lipid, which are useful for chemical industries, by changing some culture conditions. Examinations were done as a purpose of elucidating regulatory mechanism of production of these products. The kind of N-source in culture broth seemed to be important for the polyol lipid production and the initial culture pH was important for PMA production. In addition, sulfur containing amino acid, such as methionine or cysteine, and zinc ion enhanced PMA production. Copper and iron ions were found to enhance pullulan production.Mutation was performed using strain A-91 and two Met-mutants showed the same productivities as those of parent strain. These strains seemed to be useful for elucidating regulatory mechanism of production of these products.

研究了Aureobasidium sp.菌株A-91非生长细胞产生聚l -苹果酸（PMA）的过程。利用菌株A-91的非生长细胞对反应条件进行优化。在细胞制备介质中加入CuSO_4可有效制备PMA活性细胞。在最佳条件（160g/l葡萄糖和40g/l CaCO_3）下，在30‰的温度下培养5 d，菌株的未生长细胞的PMA产量为80.0g/l。另一方面，菌株A-91也可以通过改变培养条件产生对化学工业有用的普鲁兰和多元醇脂。为了阐明这些产品的生产调控机制，进行了检验。培养液中n源的种类似乎对多元醇脂的产生有重要影响，而初始培养pH对PMA的产生有重要影响。此外，含硫氨基酸（如蛋氨酸或半胱氨酸）和锌离子促进了PMA的产生。发现铜和铁离子能提高普鲁兰的产量。用菌株A-91进行突变，两个met突变体的产量与亲本菌株相同。这些菌株似乎有助于阐明这些产品的生产调控机制。