Development of T-cell vaccine for hepatitis C virus
丙型肝炎病毒T细胞疫苗的开发
基本信息
- 批准号:07557047
- 负责人:
- 金额:$ 6.14万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (A)
- 财政年份:1995
- 资助国家:日本
- 起止时间:1995 至 1997
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
We tried to develop T-cell vaccine to prevent and treat hepatitis C virus (HCV) infection. We established cytotoxic T cell clones from peripheral blood lymphocytes of two HLA B44-positive patients with chronic HCV infection that recognize HCV core antigen 88-96 as a minimal and optimal epitope. We demonstrated that positions 4 and 6 of the 9 amino acid epitope were important for the binding of the epitope and T-cell antigen receptors of the CTL clones. When amino acids at positions 4 and 7 were substituted by other amino acids, a wild type epitope for type 1b and 2a HCV (NEGLGWAGW) and a variant type for 2b HCV (NEGCGWMGW) were recognized most efficitntly by CTL slones. HCV DNA that express HCV core antigen in the tranfected mammalian cells induced HCV-specific CTL when the DNA was injected directly into the spleen of mice. Intramuscular injection of the DNA did not induce CTL in either mice or chimpanzees.
本研究试图开发T细胞疫苗来预防和治疗丙型肝炎病毒(HCV)感染。我们从两名HLAB 44阳性慢性HCV感染患者的外周血淋巴细胞中建立了细胞毒性T细胞克隆,其识别HCV核心抗原88-96作为最小和最佳表位。我们证明了9个氨基酸表位的位置4和6对于表位和CTL克隆的T细胞抗原受体的结合是重要的。当第4位和第7位氨基酸被其它氨基酸取代时,CTL克隆最有效地识别1b和2a型HCV的野生型表位(NEGLGWAGW)和2b型HCV的变异型表位(NEGCGWMGW)。将表达HCV核心抗原的HCV DNA直接注入小鼠脾脏,可诱导HCV特异性CTL。肌肉注射DNA在小鼠或黑猩猩中均未诱导CTL。
项目成果
期刊论文数量(11)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kita, Hiroto: "A helper T-cell antigen enhances generation of hepatitis C virus-specific cytotoxic T lymphocytes in vitro" Journal of Medical Virology. 45. 386-391 (1995)
Kita, Hiroto:“辅助 T 细胞抗原在体外增强丙型肝炎病毒特异性细胞毒性 T 淋巴细胞的生成”《医学病毒学杂志》。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Ando,Kazuki: "Perforin,Fas/Fas Ligand,TNF-α pathways as specific and bystander killing mechanisms of hepatitis C virus-specific human CTL" Journal of Immunology. 158. 5283-5291 (1997)
Ando,Kazuki:“穿孔素、Fas/Fas 配体、TNF-α 途径作为丙型肝炎病毒特异性人类 CTL 的特异性和旁观者杀伤机制”《免疫学杂志》158. 5283-5291 (1997)。
- DOI:
- 发表时间:
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- 影响因子:0
- 作者:
- 通讯作者:
Harase, Ichiro: "Immune response to hepatitis C virus core protein in mice" Immunology and Cell Biology. 73. 346-352 (1995)
Harase,Ichiro:“小鼠对丙型肝炎病毒核心蛋白的免疫反应”免疫学和细胞生物学。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Hiroishi,Kazumasa: "Cytotoxic T lymphocyte response and viral load in hepatitis C virus infection" Hepatology. 25(印刷中). (1997)
Hiroishi, Kazumasa:“丙型肝炎病毒感染中的细胞毒性 T 淋巴细胞反应和病毒载量”《肝病学》25(出版中)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kita, Hiroto: "HLA B44-restricted cytotoxic T lymphocyte responses to the peptides of HCV nucleoprotein residues 81-100 in patients with chronic hepatitis C" Journal of Gastroenterology. 30. 809-812 (1995)
Kita,Hiroto:“慢性丙型肝炎患者 HLA B44 限制的细胞毒性 T 淋巴细胞对 HCV 核蛋白残基 81-100 肽的反应”胃肠病学杂志。
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- 影响因子:0
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{{ truncateString('IMAWARI Michio', 18)}}的其他基金
Research on cytotoxic T cell responses to hepatitis C virus infection
细胞毒性T细胞对丙型肝炎病毒感染反应的研究
- 批准号:
14370190 - 财政年份:2002
- 资助金额:
$ 6.14万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Analysis of cellular immune responses in perihperal blood and liver tissues in HCV infection
HCV感染后外周血和肝组织细胞免疫反应分析
- 批准号:
11470136 - 财政年份:1999
- 资助金额:
$ 6.14万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Molecular and immunological study on the pathogenesis of hepatitis C
丙型肝炎发病机制的分子和免疫学研究
- 批准号:
07407015 - 财政年份:1995
- 资助金额:
$ 6.14万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Studies on the Immunopathogenesis of Viral Hepatitis C
丙型肝炎病毒免疫发病机制的研究
- 批准号:
04454241 - 财政年份:1992
- 资助金额:
$ 6.14万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
Production of antibodies to inhibit the cytotoxic activities of hepatitis C virus-specific cytotoxic T cells
生产抑制丙型肝炎病毒特异性细胞毒性 T 细胞细胞毒活性的抗体
- 批准号:
03557035 - 财政年份:1991
- 资助金额:
$ 6.14万 - 项目类别:
Grant-in-Aid for Developmental Scientific Research (B)
Molecular biological studies on a hepatoma-associated target antigen for a human killer T-cell clone
人类杀伤 T 细胞克隆的肝癌相关靶抗原的分子生物学研究
- 批准号:
01480222 - 财政年份:1989
- 资助金额:
$ 6.14万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)
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