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Establishment of a cryopreserved tissue bank for the clinical use of a llograft tissues

异体移植组织临床应用冻存组织库的建立

基本信息

批准号：
07557089
负责人：
KITAMURA Soichiro
金额：
$ 6.53万
依托单位：
Nara Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07557089/
关键词：
cryopreservation allograft valvo allograft trachea immunological regection wound healing tissue bank 同種弁移植

项目摘要

1.Experimental study cryopreserved tissue transplantation(1)Experimental cryopreserved tracheal transplantation : To clarify whether cryopreserved trachea may reduce allogenic immunoresponse, we evaluated tracheal viability and histlogical changes of cryopreserved tracheal homografts in canine and rat transplantation model without immunosuppression. Our esxperimental results showed excellent long-term viability of cryopreserved grafts. The epithelium, smooth muscle and cartilage were retained after transplantation without immunosuppression. Cryopreservation reduced the allogenic response of the tracheal in experimental models.(2)Effect of cryopresarvation on fibroblast growth factor (FGF) gene expression in cryopreserved rat aortic homografts : To elarify whether cryopreserved aortic homograft may produce FGF in recipients, cryopreserved rat aortic homegrafts were implanted heterotopically into allogenic recipient rats. Reverse transcriptase-polymerase chain reaction with a rat a-FGF complementary DNA probe was used to assess the expression of a-FGF mRNR.Both fresh and cryporeserved grafts exhibited significant expression of a-FGF mRNA 4 weeks after transplantation. Preservation of gene expression for a-FGF suggests a capability for stimulation of topical wound healing.2.Trial to organize a cryopreserved allograft bankFor the systematical clinical use of the cryopreserved allograft tissues, we made an initial trial to organize a network and banking system of cryopreserved allograft valve in Japan. Allograft valves could be procured from younger donors and in shorter warm ischemic period due to this network system. The network we made could provide allograft valves of excellent qualty. The uniform data base of the cryopreserved thoracic and cardiovascular tissues was made for the aim of country-wide tissue sharing.

1.冻存组织移植的实验研究（1）冻存气管移植的实验研究：为了明确冻存气管是否会降低同种异体的免疫反应，我们在犬和大鼠无免疫抑制的移植模型中评价了冻存同种气管移植物的存活率和组织学变化。我们的实验结果表明，冷冻保存的移植物具有良好的长期存活能力。移植后未使用免疫抑制剂，上皮、平滑肌和软骨得以保留。在实验模型中，冷冻保存降低了气管的同种异体反应。（2）深低温保存对大鼠同种异体主动脉成纤维细胞生长因子（FGF）基因表达的影响：将深低温保存的大鼠同种异体主动脉移植物异位植入同种异体受体大鼠体内，观察受体是否能产生FGF。用大鼠a-FGF互补DNA探针进行逆转录聚合酶链反应（RT-PCR）检测a-FGF mRNR的表达。保存a-FGF的基因表达提示了刺激局部伤口愈合的能力。2.尝试组织冷冻保存的同种异体移植物库为了系统地临床应用冷冻保存的同种异体移植物组织，我们在日本进行了初步尝试，组织冷冻保存的同种异体移植物瓣膜的网络和库系统。由于这个网络系统，同种异体瓣膜可以从更年轻的供体和更短的热缺血期获得。我们制作的人工瓣膜网可以提供优质的同种异体瓣膜。建立统一的胸心血管组织深低温保存数据库，实现全国范围内的组织共享。