Oxydant Stress in the Cardiomyocyte and Its Roles on the Cellular Signaling

组织氧张力是有氧代谢的重要决定因素。更密切地说，细胞表面和线粒体之间的张力梯度可能是ATP转换的更直接的调节剂。虽然已经有了假设，但尚未测量出每个细胞内氧张力的细胞内分布水平。在这项研究中，我们专注于开发一种新的系统，在该系统中，细胞内氧分布在一个孤立的单个肌细胞中以实时的方式二维可视化。该方法是通过在共聚焦显微镜上对一种亲磷蛋白衍生物在激光激发下的局部磷光猝灭进行定量而得到的。我们已经开发了一种方法，通过该方法将卟啉衍生物注射到单个肌细胞中，而不会造成显著的细胞损伤。该研究目前扩展到配置激光激发的设置和在共聚焦显微镜和附带的计算机分析仪中发射荧光的数据采集。这些数据可能提供有氧和无氧代谢的基本信息，以及氧分子作为瞬时信号载体的亚细胞作用。

The tissue oxygen tension is important determinant of the aerobic metabolism. More closely, the tension gradient between the cell surface and the mitochondria might be the more direct regulator of the ATP turnover. Though it has been assumed, the level of intracellular distribution of the oxygen tension in each cell has not been measured yet. In this study, we focused to develop a new system in which the intracellular oxygen distribution was two-dimensionally visualized in a real-time fashion in an isolated single myocyte. The method was derived from the quantification of the regional phosphorescence queching of a phorphilin derivative during the laser excitation on the confocal microscopic apparatus. We have developed a methodology by which the phorphylin derivative was injected into the individual myocyte without significant cellular damage. The study is currently extended to configure the setting of the laser excitation and the data acquisition of the emitted fluorescence in the confocal microscope and the attached computer-based analyzer. The data might provide the essential information of aerobic as well as anaerobic metabolism and the subcellular roles of the oxygen molecule as a transient signal carrier.