Development of Superasialoglycoprotein models with combined functions of Liver-cells Recognition and Drugs-inclusion

在药物和基因传递中，靶向的时空控制是最重要的课题，通过设计细胞可识别和释放可控的聚合物载体，本研究设计并评价了能够识别肝细胞并结合化学物质和DNA试剂的寡糖载体聚合物。发现乳糖半乳糖载1，4-葡萄糖的聚苯乙烯衍生物（PVLA）是一种具有较高的肝细胞识别能力和体内外药物包合能力的药物载体。还合成了多糖缀合物聚合物，并将其表征为肝癌细胞（HepG 2）的肝细胞特异性DNA载体。制备了赖氨酸主链和透明质酸侧链，并对它们作为DNA载体的应用前景进行了评价。

In drugs and gene delivery, spatio-temporal control of targeting is the most importantsubject by design of cell-recognizable and release-controllable polymeric carriers.In this research project, oligosaccharide-carrying polymers which are capable of recognizing liver cells and incorporating chemicals and DNA reagents are designed and estimated. It was found out that lactose galactose (1, 4-glucose) -carrying polystyrene derivative (PVLA) is one of the best candidates which have higher capacityof hepatocyte-recognition as well as drugs inclusion by in vitro and in vivo assays.Poly-lysine, polysaccharide conjugates polymers were also synthesized and characterised as hepatocyte-specific DNA carrier to heptoma cell (HepG2).Copolymers consisiting of poly-L-lysine backbone and hyaluronic acid side chain were also prepared and evaluated in viewpoint at sinusoidal endothelial cell tergeting as DNA carriers.