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Development of Superasialoglycoprotein models with combined functions of Liver-cells Recognition and Drugs-inclusion

具有肝细胞识别和药物包涵功能的超级唾液酸糖蛋白模型的开发

基本信息

批准号：
07558212
负责人：
AKAIKE Toshihiro
金额：
$ 3.58万
依托单位：
Tokyo Institute of Technology
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

项目摘要

In drugs and gene delivery, spatio-temporal control of targeting is the most importantsubject by design of cell-recognizable and release-controllable polymeric carriers.In this research project, oligosaccharide-carrying polymers which are capable of recognizing liver cells and incorporating chemicals and DNA reagents are designed and estimated. It was found out that lactose galactose (1, 4-glucose) -carrying polystyrene derivative (PVLA) is one of the best candidates which have higher capacityof hepatocyte-recognition as well as drugs inclusion by in vitro and in vivo assays.Poly-lysine, polysaccharide conjugates polymers were also synthesized and characterised as hepatocyte-specific DNA carrier to heptoma cell (HepG2).Copolymers consisiting of poly-L-lysine backbone and hyaluronic acid side chain were also prepared and evaluated in viewpoint at sinusoidal endothelial cell tergeting as DNA carriers.

在药物和基因传递中，靶向的时空控制是最重要的课题，通过设计细胞可识别和释放可控的聚合物载体，本研究设计并评价了能够识别肝细胞并结合化学物质和DNA试剂的寡糖载体聚合物。发现乳糖半乳糖载1，4-葡萄糖的聚苯乙烯衍生物（PVLA）是一种具有较高的肝细胞识别能力和体内外药物包合能力的药物载体。还合成了多糖缀合物聚合物，并将其表征为肝癌细胞（HepG 2）的肝细胞特异性DNA载体。制备了赖氨酸主链和透明质酸侧链，并对它们作为DNA载体的应用前景进行了评价。

项目成果

期刊论文数量（12）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

C.S.Cho, Y.I.Jeong, T.Ishihara, R.Takei, J.U.Park, K.H.Park, A.Maruyama, T.Akaike: "Simple preparation of nanoporticles coated with carbohydrate-carrying polymers" Biomaterials.18. 323-326 (1997)

C.S.Cho、Y.I.Jeong、T.Ishihara、R.Takei、J.U.Park、K.H.Park、A.Maruyama、T.Akaike：“简单制备涂有碳水化合物携带聚合物的纳米颗粒”Biomaterials.18。

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

Jeong-Ung Park: "Preparation of dendric graft copolymer consisting of poly (L-lysine) and arabinogalactan as a hepatocyte specific DNA carrier" Preparative Biochemistry & Biotechnology. in press.

Jeong-Ung Park：“制备由聚（L-赖氨酸）和阿拉伯半乳聚糖组成的树状接枝共聚物作为肝细胞特异性 DNA 载体”制备生物化学

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

Shoichiro ASAYAMA,Masayuki NOGAWA,Yoshiyuki TAKEI,Toshihiro AKAIKE,and Atushi MARUYAMA: "Synthesis of Novel Polyampholyte Comb-Type Copolymers Consisiting of a Poly (L-lysine) Backbone and Hyaluronic Acid Side Chains for a DNA Carrier" Bioconjugate Chem.

Shoichiro ASAYAMA、Masayuki NOGAWA、Yoshiyuki TAKEI、Toshihiro AKAIKE 和 Atushi MARUYAMA：“由聚（L-赖氨酸）主链和用于 DNA 载体的透明质酸侧链组成的新型聚两性电解质梳型共聚物的合成”Bioconjugate Chem。