Studies on the Biosynthetic Method and Improvement of Production of Novel Polysaccharides from Living Wastes
Studies on the Biosynthetic Method and Improvement of Production of Novel Polysaccharides from Living Wastes
批准号:
07558245
负责人:
TOKURA Seiichi
金额:
$3.01万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (A)
财政年份:
1995
资助国家:
日本
项目状态:
已结题
起止时间:
1995 至 1997
中文摘要
采用含葡萄糖培养基对木动杆菌进行反复传代培养,制备n -乙酰氨基葡萄糖(GlcNAc)残基诱导细菌纤维素(2-4摩尔%)。由于以葡萄糖胺或半乳糖胺作为碳源代替葡萄糖nac可使GlcNAc残渣掺入相似水平,但甘露糖胺效果不佳,因此建议脱氨酶参与GlcNAc残渣掺入。考虑到氨离子是脱氨酶的最终产物,采用一种新型的好氧旋转培养装置,将葡萄糖和铵盐成功地应用于葡萄糖nac与细菌纤维素的结合。用浅盘培养器成功地制备了新型纤维性细菌纤维素。这些纤维被证明具有比棉花更高的抗拉强度。
英文摘要
Actobacter xylinum has been subcultured repeatedly to prepare N-acetylglucosamin (GlcNAc) residue induced bacterial cellulose (2-4 mole%) applying GlcNAc containing glucose medium. As a similar level of GlcNAc residue incorporation has been made by applying glucosamin or galactosamin as carbonsource instead of GlcNAc, but mannosamin was not effective, participation of deaminase was suggested on the GlcNAc residue incorporation. Since ammonium ion was assumed to be final product by deaminase, gulucose and ammonium salts were applied successfully to incorporation of GlcNAc into bacterial cellulose by applying a new type of aerobic rotatory cultivator. A shallow pan cultivator has been made to prepare fibrous novel bacterial celluloses successfully. These fibers were shown to have higher tensile strength than that of cotton.
期刊论文(14)
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K. Inata, S. Tokura他3名: "Utilization of DNA as functional materials : preparation of filters containing DNA insolubilized with alginic acid gel" Int. J. Biol. Mocromol.18. 149-150 (1996)
K. Inata、S. Tokura 和其他 3 人:“DNA 作为功能材料的利用:含有藻酸凝胶不溶性 DNA 的过滤器的制备”J. Biol.149-150 (1996)。
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Kitamura,H., 他4名: "Marked effect of DNA on collagen fibrillogenesis in vitro" Int.J.Biol.Macromol.20. 241-244 (1997)
Kitamura, H. 和其他 4 人:“DNA 对体外胶原纤维生成的显着影响”Int.J.Biol.Macromol.20 (1997)。
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Shirai, A., 他3名: "Preparation of a novel(1→4)-β-D-glycan by Acetobacter xylinum--a proposed mechanism for incorporation of a N-acetylglucosamine residue into bacterial cellulose" Carbohydrate Polymers. 32. 223-227 (1997)
Shirai, A. 和其他 3 人:“木醋杆菌制备新型 (1→4)-β-D-聚糖——一种将 N-乙酰氨基葡萄糖残基掺入细菌纤维素的机制”碳水化合物聚合物 32。 223-227 (1997)
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戸倉清一: "A sulfated chitin,SCM-chitin III, inhibits the clearance of human erythrocytes from the blood circulation in erythrocyte-transfused SCID mice" Immunopharmacology. 29. 65-71 (1995)
Seiichi Togura:“硫酸化几丁质,SCM-几丁质 III,抑制红细胞输注 SCID 小鼠血液循环中的人红细胞清除”《免疫药理学》29. 65-71 (1995)。
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Mori,T., 他7名: "Effects of chitin and its derivatives on the proliferation and cytokine production of fibroblasts in vitro" Biomaterials. 18. 947-951 (1997)
Mori, T. 和其他 7 人:“几丁质及其衍生物对体外成纤维细胞增殖和细胞因子产生的影响”生物材料 18. 947-951 (1997)。
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共 13 条
Study of Chitin and Chitosan for Bio-medical Materials Application
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批准号:14350504
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项目类别:Grant-in-Aid for Scientific Research (B)
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资助金额:$9.66万
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财政年份:2002
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负责人:TOKURA Seiichi
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依托单位:
STUDIES ON THE DEVELOPMENT OF VARIOUS TYPES OF DRUG DERIVERY SYSTEM USING CHITIN DERIVATIVES
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批准号:02555183
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项目类别:Grant-in-Aid for Developmental Scientific Research (B)
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资助金额:$11.39万
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财政年份:1990
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负责人:TOKURA Seiichi
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依托单位:
Studies on the Syntheses of Bioactive Chitin Derivatives.
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批准号:60430025
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项目类别:Grant-in-Aid for General Scientific Research (A)
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资助金额:$13.44万
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财政年份:1985
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负责人:TOKURA Seiichi
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依托单位:
海外基金