Studies on the Biosynthetic Method and Improvement of Production of Novel Polysaccharides from Living Wastes

生活垃圾新型多糖的生物合成方法及工艺改进研究

• 批准号: 07558245
• 负责人: TOKURA Seiichi
• 金额: $ 3.01万
• 依托单位: HOKKAIDO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07558245/
• 关键词: Bacterial cellulose; Acetobacter xylinum; N-acetylglucosamin; Cultivation; A novel polysaccharides; Direct filamentation; Aerobic rotatory cultivator; Shallow pan cultivator; バクテリアセルロース; グルコース; 静置培養; 回転通気培養; アミノ糖残基; 塩化アンモニウム; 燐酸化アミノ酸; カルボキシ

Actobacter xylinum has been subcultured repeatedly to prepare N-acetylglucosamin (GlcNAc) residue induced bacterial cellulose (2-4 mole%) applying GlcNAc containing glucose medium. As a similar level of GlcNAc residue incorporation has been made by applying glucosamin or galactosamin as carbonsource instead of GlcNAc, but mannosamin was not effective, participation of deaminase was suggested on the GlcNAc residue incorporation. Since ammonium ion was assumed to be final product by deaminase, gulucose and ammonium salts were applied successfully to incorporation of GlcNAc into bacterial cellulose by applying a new type of aerobic rotatory cultivator. A shallow pan cultivator has been made to prepare fibrous novel bacterial celluloses successfully. These fibers were shown to have higher tensile strength than that of cotton.

采用含N-乙酰氨基葡萄糖（GlcNAc）的葡萄糖培养基，对木醋杆菌进行反复继代培养，制备2-4摩尔%的GlcNAc残基诱导的细菌纤维素。由于使用葡糖胺或半乳糖胺作为碳源代替GlcNAc，可以获得相似水平的GlcNAc残基掺入，但甘露糖胺无效，因此建议脱氨酶参与GlcNAc残基掺入。由于铵离子被认为是脱氨酶的最终产物，因此，通过应用新型好氧旋转培养器，将古洛糖和铵盐成功地用于将GlcNAc掺入细菌纤维素。利用浅盘培养器成功地制备了纤维状的新型细菌纤维素。这些纤维被证明具有比棉更高的拉伸强度。

项目成果

K. Inata, S. Tokura他3名: "Utilization of DNA as functional materials : preparation of filters containing DNA insolubilized with alginic acid gel" Int. J. Biol. Mocromol.18. 149-150 (1996)

K. Inata、S. Tokura 和其他 3 人：“DNA 作为功能材料的利用：含有藻酸凝胶不溶性 DNA 的过滤器的制备”J. Biol.149-150 (1996)。

Kitamura,H., 他4名: "Marked effect of DNA on collagen fibrillogenesis in vitro" Int.J.Biol.Macromol.20. 241-244 (1997)

Kitamura, H. 和其他 4 人：“DNA 对体外胶原纤维生成的显着影响”Int.J.Biol.Macromol.20 (1997)。

Shirai, A., 他3名: "Preparation of a novel(1→4)-β-D-glycan by Acetobacter xylinum--a proposed mechanism for incorporation of a N-acetylglucosamine residue into bacterial cellulose" Carbohydrate Polymers. 32. 223-227 (1997)

Shirai, A. 和其他 3 人：“木醋杆菌制备新型 (1→4)-β-D-聚糖——一种将 N-乙酰氨基葡萄糖残基掺入细菌纤维素的机制”碳水化合物聚合物 32。 223-227 (1997)

戸倉清一: "A sulfated chitin,SCM-chitin III, inhibits the clearance of human erythrocytes from the blood circulation in erythrocyte-transfused SCID mice" Immunopharmacology. 29. 65-71 (1995)

Seiichi Togura：“硫酸化几丁质，SCM-几丁质 III，抑制红细胞输注 SCID 小鼠血液循环中的人红细胞清除”《免疫药理学》29. 65-71 (1995)。

Mori,T., 他7名: "Effects of chitin and its derivatives on the proliferation and cytokine production of fibroblasts in vitro" Biomaterials. 18. 947-951 (1997)

Mori, T. 和其他 7 人：“几丁质及其衍生物对体外成纤维细胞增殖和细胞因子产生的影响”生物材料 18. 947-951 (1997)。