Cathepsin E-its role in the processing of biologically active peptides and gene expression
Cathepsin E-its role in the processing of biologically active peptides and gene expression
批准号:
07640900
负责人:
KAGEYAMA Takashi
金额:
$1.47万
依托单位:
依托单位国家:
日本
项目类别:
Grant-in-Aid for Scientific Research (C)
财政年份:
1995
资助国家:
日本
项目状态:
已结题
起止时间:
1995 至 1996
中文摘要
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英文摘要
1.Cathepsin E processed the precursors of the neurotensin, neuromedin, xenopsin, and related bioactive peptides. The common structural features that specified the processing sites were found in the carboxyl-terminal sequences of the active peptides of these precursors ; namely, the sequence Pro-X-X'-hydrophobic amino acid was found at positions P4 through P1.2.Cathepsin E processed invariant chain to CLIP.The sequence of the P4 through P1 of the cleavage site was Pro-Leu-Leu-Met, and this was consistent with the consensus sequence that is recognized by cathepsin E.3.A new method for the assay of cathepsin E was developed. The method was based on the specific activity of cathepsin E against substance P.
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Kageyama,T.,et al.: "Potential sites for processing of the human invariant chain by cathepsins D and E" Biochemical Biophysical Research Communications. 223. 549-553 (1996)
Kageyama,T.,et al.:“组织蛋白酶 D 和 E 处理人类不变链的潜在位点”生化生物物理研究通讯。
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通讯作者:
Kageyama, T., et al.: "Isolation, characterization and structure of procathepsin E and cathepsin E from the gastric mucosa of guinea pig" Advances of Experimental Biology and Medicine. 362. 211-221 (1995)
Kageyama, T. 等人:“豚鼠胃粘膜组织蛋白酶 E 和组织蛋白酶 E 的分离、表征和结构”实验生物学和医学进展。
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Kageyama, T., et al.: "Determination of cathepsins D and E in various tissues and cells of rat, monkey, and man by the assay with β-endorphin and substance P as substrates" Zoological Science. 13. 693-698 (1996)
Kageyama, T. 等人:“通过以 β-内啡肽和 P 物质为底物的测定法测定大鼠、猴子和人的各种组织和细胞中的组织蛋白酶 D 和 E”《动物学科学》。 1996)
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Kageyama, T.: "Procathepsin E and Cathepsin E" Methods in Enzymology. 248. 120-136 (1995)
Kageyama, T.:“组织蛋白酶 E 和组织蛋白酶 E”酶学方法。
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作者:
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通讯作者:
T.Kageyama: "Procathepsin E and Cathepsin E" Advances in Experimental Medicine and Biology. 306. 211-221 (1995)
T.Kageyama:“组织蛋白酶 E 和组织蛋白酶 E”实验医学和生物学进展。
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共 18 条
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