Development and Application of a Novel Bioreactor with Loofa (Luffa cylindrica) Sponge as a Carrier For Cell Immobilization.

以丝瓜络海绵为细胞固定化载体的新型生物反应器的研制及应用。

• 批准号: 07650946
• 负责人: OGBONNA James chukwuma
• 金额: $ 1.34万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07650946/
• 关键词: Loofa spong; Cell Immobilization; Immobilization carrier; Flocculating cells; Non-flocculating cells; Filamentous cells; Aerobic fermentation process; Anaerobic fermentation process; 充填層; アミラーゼ生産; 細胞固定化; 物質生産

Efficient immobilization of flocculating cells can be achieved by simply inoculating a reactor containing a bed of the loofa sponge with the cell suspension. The cells were trapped inside the lattice of the sponge and grew to high cell densities. In the case of non-flocculating cells, flocculation was induced by addition of flocculating agent (chitosan) and the flocculated cells were efficiently immobilized as described above. Fungi was immobilized by inoculating either spores or homogenized hyphae into a reactor containing a bed of the loofa sponge. The spores germinated inside the lattice structure of the sponge and the resulting hyphae were entangled (immobilized) on the sponge.Although mass transfer was better in a fixed bed made of a single cylindrical sponge, the amounts of sponge per unit reactor volume and the immobilized cells per unit sponge were higher when sliced loofa sponges were used for bed construction. Thus cylindrical or sliced loofa sponges should be used for aerobi … More c or anaerobic fermentation processes, respectively.As examplex of application to anaerobic fermentation, immobilized flocculating (Saccharomyces cerevisiae) or non-flocculating (Candida brassicae) was used for continuous ethanol production from molasses and sucrose medium. The processes were stable for long periods of time. In the case of aerobic processes, long term continuous glucoamylase production by Aspergillus awamori immobilized on loofa sponge was possible. Furthermore, continuous direct ethanol production from starch was achieved by using a combination of immobilized Aspergillus awamori and Saccharomyces cerevisiae.The above results have shown that representative flocculating and non-flocculating fermentation microorganisms (yeast, bacteria and fungi) can be efficiently immobilized on loofa sponge. Furthermore, by using either the cylindrical or sliced loofa sponge, suitable fixed beds for aerobic or anaerobic fermentation processes can be constructed. It can be thus concluded that bioreactor with loofa sponges has great potential for application in a wide range of fermentation processes. Less

只需将细胞悬浮液接种到含有丝瓜络床的反应器中，即可实现絮凝细胞的有效固定。细胞被困在海绵晶格内并生长至高细胞密度。在非絮凝细胞的情况下，通过添加絮凝剂(壳聚糖)来诱导絮凝，并且如上所述，絮凝的细胞被有效地固定化。通过将孢子或均质菌丝接种到含有丝瓜络海绵床的反应器中来固定真菌。孢子在海绵的晶格结构内萌发，产生的菌丝缠绕（固定）在海绵上。虽然在由单个圆柱形海绵制成的固定床中传质较好，但当使用切片丝瓜络海绵构建床时，每单位反应器体积的海绵量和每单位海绵的固定细胞量更高。因此，圆柱形或切片丝瓜海绵应分别用于需氧或厌氧发酵过程。作为厌氧发酵应用的示例，固定絮凝（酿酒酵母）或非絮凝（甘蓝假丝酵母）用于从糖蜜和蔗糖培养基连续生产乙醇。这些过程在很长一段时间内保持稳定。在有氧过程的情况下，固定在丝瓜络海绵上的泡盛曲霉可以长期连续生产葡糖淀粉酶。此外，通过固定化泡盛曲霉和酿酒酵母的组合，实现了淀粉连续直接生产乙醇。上述结果表明，代表性絮凝和非絮凝发酵微生物（酵母、细菌和真菌）可以有效地固定在丝瓜络上。此外，通过使用圆柱形或切片丝瓜络海绵，可以构建适合需氧或厌氧发酵过程的固定床。由此可见，丝瓜络生物反应器在广泛的发酵过程中具有巨大的应用潜力。较少的