Fate of a specific microorganism in soil.

土壤中特定微生物的命运。

• 批准号: 07660085
• 负责人: NISHIYAMA Masaya
• 金额: $ 1.28万
• 依托单位: Yamguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660085/
• 关键词: tomato bacterial wilt; Burkholderia solanacearum; rhizosphere; rhizobacteria; whole-cell hybridization; ribosome staining; whole-cell hybridization; 土壌微生物; 根面微生物; 蛍光性Pseudomonas

(1) Detection of the cells of tomato bacterial wilt agent, Burkholderia solanacearum, by whole-cell hybridization : Ribosomal DNA sequence specific for B.solanacearum was investigated using DNA database. By using the detection probe, cells of B.solanacearum in pure culture can be stained specifically. In addition, the cells can be detected in tomato rhizoplane.(2) Behavior of B.solanacearum in tomato rhizosphere.1) Soil moisture condition and soil temperature condition were investigated for controlling tomato bacterial wilt when B.solanacearum was inoculated to soil.2) Population dynamics of B.solanacearum in tomato soil were investigated under the conditions obtained in 1) by plate counting. In the suppressive soil used, growth of B.solanacearum was suppressed in rhizosphere and rhizoplane and within the roots more than in the induced soil. No clear difference was observed in non-rhizosphere soil between the two soils.

(1)番茄青枯病病原菌细胞的全细胞杂交检测：利用DNA数据库对青枯病病原菌的核糖体DNA序列进行分析。利用该检测探针，可以对青枯菌纯培养细胞进行特异性染色。(2)青枯菌在番茄根际的行为1)土壤接种青枯病的土壤水分条件和土壤温度条件2)采用平板计数法研究了青枯菌在番茄土壤中的种群动态。在抑制土壤中，青枯菌在根际、根面和根内的生长受到的抑制比在诱导土壤中的抑制要大。在非根际土壤中，两种土壤的差异不明显。