Fate of a specific microorganism in soil.

(1)全细胞杂交技术检测番茄青枯病菌的研究：利用DNA数据库对B.solanacearum的特异核糖体DNA序列进行分析。利用该检测探针可对纯培养的青枯B菌细胞进行特异性染色。此外，在番茄根面也能检测到该细胞。(2)研究了青枯B.solanacearum在番茄根际的行为：1）研究了接种青枯B.solanacearum后土壤水分和温度条件对防治番茄青枯病的影响; 2）在上述条件下，采用平板计数法研究了青枯B.solanacearum在番茄土壤中的种群动态。在抑制土中，青枯B菌在根际、根面和根内的生长受到抑制的程度大于在诱导土中。两种土壤的非根际土壤中的差异不明显。

(1) Detection of the cells of tomato bacterial wilt agent, Burkholderia solanacearum, by whole-cell hybridization : Ribosomal DNA sequence specific for B.solanacearum was investigated using DNA database. By using the detection probe, cells of B.solanacearum in pure culture can be stained specifically. In addition, the cells can be detected in tomato rhizoplane.(2) Behavior of B.solanacearum in tomato rhizosphere.1) Soil moisture condition and soil temperature condition were investigated for controlling tomato bacterial wilt when B.solanacearum was inoculated to soil.2) Population dynamics of B.solanacearum in tomato soil were investigated under the conditions obtained in 1) by plate counting. In the suppressive soil used, growth of B.solanacearum was suppressed in rhizosphere and rhizoplane and within the roots more than in the induced soil. No clear difference was observed in non-rhizosphere soil between the two soils.