Design of Lignin-Based Biofunctional Materials and Their Applications

木质素基生物功能材料的设计及其应用

• 批准号: 07660219
• 负责人: FUNAOKA Masamitsu
• 金额: $ 1.28万
• 依托单位: Mie University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07660219/
• 关键词: Lignin; Lignophenol derivatives; Phase-separation process; Protein; Enzyme immobilization; Bioreactor; Hydrophobic interaction; Biofunctional materials; フェノール化; フェノール誘導体; 相分離; 吸着; 機能性高分子

Highly phenolic lignin derivatives (lignophenol derivatives) with different phenolic functionalities were synthesized directly from native lignins, through the phase-separation process using phenol derivatives and concentrated acid. The relationships between the structures of lignophenol derivatives and their functions for proteins were discussed.Lignophenol derivatives had much higher affinities for proteins, compared with conventional lignins : -10 and -100 times higher in lignomonophenols and lignopolyphenols, respectively. The protein affinity was significantly affected by the substitution pattern of phenolic hydroxyl groups and was decreased by the introduction of hydrophobic alkyl substituents. The protein adsorbing capacities of lignophenol derivatives differed with the species from which they were derived. The maximum protein adsorption was achieved at the isoelectric points of used proteins. The lignophenol derivative-protein complex was rapidly and perfectly separated into lignin and protein moieties in aprotic solvents, although it was very stable at any pH in aqueous solution.The enzymes immobilized on lignophenol derivatives retained high activity : especially an enzyme on lignocresol comparable to a native one. The activity patterns had good correlations between native and immobilized enzymes.

以天然木质素为原料，经苯酚衍生物和浓酸相分离，直接合成了具有不同酚功能的高酚木质素衍生物（木质素酚衍生物）。讨论了木质素酚衍生物的结构与蛋白质功能之间的关系。与传统木质素相比，木质素酚衍生物对蛋白质的亲和力要高得多：木质素单酚和木质素多酚的亲和力分别高-10倍和-100倍。苯酚羟基的取代模式显著影响蛋白质的亲和力，疏水性烷基取代基的引入降低了蛋白质的亲和力。木质素酚衍生物的蛋白质吸附能力因其来源的物种而异。在所用蛋白质的等电点处，蛋白质吸附量最大。木质素酚衍生物-蛋白质复合物在非质子溶剂中迅速而完美地分离为木质素和蛋白质部分，尽管它在任何pH值的水溶液中都非常稳定。固定在木质素酚衍生物上的酶保持了较高的活性，特别是木质素甲酚上的酶与天然酶相当。天然酶和固定化酶的活性模式具有良好的相关性。

项目成果

船岡正光: "天然リグニンからのポリマー合成" 高分子加工. 46. (1997)

Masamitsu Funaoka：“天然木质素的聚合物合成”聚合物加工 46。（1997）

M.Funaoka, H.Ioka, T.Hosho and Y.Tanaka: "Synthesis of Phenolic Lignin Derivatives from Native Lignins and Their Functionality Control by the Participation of Calpha-Phenolic Nuclei" J.Network Polymer. 17. 121-130 (1996)

M.Funaoka、H.Ioka、T.Hosho 和 Y.Tanaka：“从天然木质素合成酚醛木质素衍生物及其通过 Cα-酚核的参与控制功能”J.Network Polymer。

井岡浩之: "側鎖Ca位フェノール核の関与によるリグノフェノール誘導体の分子構造および機能制御" 熱硬化性樹脂討論会講演集. 45. 141-144 (1995)

Hiroyuki Ioka：“通过侧链 Ca 位置的苯酚核的参与控制木质素酚衍生物的分子结构和功能”热固性树脂研讨会论文集 45. 141-144 (1995)。

H.T.Chen, M.Funaoka and Y.Z.Lai: "Attempts to Understand the Nature of Phenolic and Etherified Components of Wood Lignin" Wood Sci.Technol.(in press). (1997)

H.T.Chen、M.Funaoka 和 Y.Z.Lai：“尝试了解木材木质素酚类和醚化成分的性质”《木材科学技术》（出版中）。

船岡正光: "機能性フェノール系リグニン素材の設計と合成に関する研究" ネットワークポリマー講演集. 46. 149-152 (1996)

Masamitsu Funaoka：“功能性酚醛木质素材料的设计与合成研究”网络聚合物讲座46。149-152（1996）。