Functional analysis of roles of Pax-1 during vertebral column development

Pax-1在脊柱发育过程中的作用的功能分析

• 批准号: 07670003
• 负责人: KOSEKI Haruhiko
• 金额: $ 1.28万
• 依托单位: Chiba University, School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670003/
• 关键词: vertebral column; dorsovental axis; Pax-1; sclerotome; notochord; 体節分化; コラーゲン(α1)

Pax-1 is transcriptional activator containing paired-box as DNA binding domain. From the analysis of Pax-1 deficient mice, Pax-1 was proved to be required for the generation of ventral structures of axial skeleton. To gain further insights for Pax-1 function, I have generated mice over expressing Pax-1 protein. In this mouse, development of caudal vertebral distal region of rib were affected. This result indicates that down regulation of Pax-1 is also important for the morphogenesis of axial skeleton.

PAX-1是包含成对框作为DNA结合结构域的转录激活剂。从对PAX-1缺乏小鼠的分析中，事实证明，PAX-1是轴向骨骼的腹侧结构所必需的。为了获得对PAX-1功能的进一步见解，我在表达PAX-1蛋白上产生了小鼠。在这只小鼠中，肋骨的尾椎远端区域的发展受到影响。该结果表明，向下调节PAX-1对于轴向骨骼的形态发生也很重要。

项目成果

Muller,T.S.: "Expression of avian Pax1 and Pax9 is intrinsically regulated in the pharyngeal endoderm,but depends on environmental influences in the paraxial mesoderm." Dev.Biol.178. 403-417 (1996)

Muller, T.S.：“禽类 Pax1 和 Pax9 的表达在咽内胚层中受到内在调节，但取决于轴旁中胚层的环境影响。”

Ebensperger, C., Wilting, J., Brand-Saberi, B., Mizutani, Y., Christ, B., Balling, R.and Koseki, H.: "Pax-1, a regulator of sclerotome development is induced by notochord and floor plate signals in avian embryos." Anat.Embryol.191. 297-310 (1995)

Ebensperger, C.、Wilting, J.、Brand-Saberi, B.、Mizutani, Y.、Christ, B.、Balling, R. 和 Koseki, H.：“Pax-1 是菌核发育的调节因子

Kaneko, K., Taniguchi, M.and Koseki, H.: "Production of monoclonal antibodies against quail Pax-1." Hybridoma. 15. 83-86 (1996)

Kaneko, K.、Taniguchi, M. 和 Koseki, H.：“针对鹌鹑 Pax-1 的单克隆抗体的生产。”

Kaneko,K.: "Production of monoclonal antibodies against quail Pax-1." Hybridoma. 15. 83-86 (1996)

Kaneko,K.：“针对鹌鹑 Pax-1 的单克隆抗体的生产。”

Neubueser, A.: "Characterization and developmental expression of Pax-9, a paired box containing gene related to Pax-1." Developmental Biology.170. 701-716 (1995)

Neubueser, A.：“Pax-9 的表征和发育表达，Pax-9 是一个包含 Pax-1 相关基因的配对盒。”