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RESEARCH OF ANTI-HIV ACTIVITY SUBSTANCES IN LABIATAE PLANTS,AROMATIC PLANTS AND STUDIES ON THE ACTION MECHANISM

唇形科植物、芳香植物抗HIV活性物质的研究及作用机制研究

基本信息

批准号：
07670480
负责人：
YAMASAKI Katsuhiro
金额：
$ 0.77万
依托单位：
OSAKA PREFECTURAL INSTITUTE OF PUBLIC HEALTH
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670480/
关键词：
HIV AIDS green perilla lemon balm bazil cinnamon self heal grapefruit mint winter savory

项目摘要

We have screened and identified compounds that inhibit the replication of HIV-1 from natural products and defined the mechanisms of the anti-HIV-1 activity of the hit. We found some Labiatae plants exhibiting anti-HIV activity. We screened several aromatic plants (herbs) belonging to Labiatae, and searched for the active substances.About 40 herbs belonging to Labiatae used in Japan were extracted in the order acetone, ethanol, 70% ethanol and water. Each extract was investigated for inhibition of the replication of HIV-1 (LAV-1). Acetone and ethanol extracts of all the samples showed no activity, but the 70% ethanol extract showed light activity. Most of the water extract showed the anti-HIV-1 activity. Especially, Prunella vulgaris, Ocimum basilicum L.cv cinnamon, Melissa officianalis L.and Perilla frutescens Britt. var. acta KUdo showed very potent activity at below the concentration of 16mg/ml. By use column chromatography, we are searching for common anti-HIV-1 active substances in these herbs.Replication of the fresh isolated HIV-1 strain was inhibited by some extracts. The extracts weakly inhibited the reverse transcriptase activity of HIV-1. All the extracts suppressed giant cell formation in co-cultures of HIV-1-infected and non-infected Molt-4 cells, suggesting that these compounds can inhibit virus adsorption or fusion.Through our study, we found many herbs with anti-HIV-1 activity in vitro. It is vary meaningful that herbs belonging to Labiatae plant, reasonable and easy to cultivate, show very potent anti-HIV-1 activity. It is very important to search for the common active substance in these Labiatae plants.

我们已经从天然产物中筛选和鉴定了抑制HIV-1复制的化合物，并确定了HIT抗HIV-1活性的机制。我们发现了一些具有抗HIV活性的唇形科植物。我们筛选了几种唇形科芳香植物(草本植物)，并寻找其活性物质。按丙酮、乙醇、70%乙醇和水的顺序提取了约40种在日本使用的唇形科草本植物。研究每种提取物对HIV-1(LAV-1)复制的抑制作用。所有样品的丙酮和乙醇提取物均无活性，但70%乙醇提取物有轻度活性。大部分水提物具有抗HIV-1活性。特别是夏枯草、巴西罗勒、肉桂、梅丽莎和紫苏。瓦尔Acta Kudo在低于16 mg/ml的浓度下表现出很强的活性。通过柱层析，我们在这些草药中寻找共同的抗HIV-1活性物质。提取物对HIV-1逆转录酶活性有微弱的抑制作用。所有提取物均抑制HIV-1感染和未感染的Molt-4细胞共培养中巨细胞的形成，表明这些化合物能够抑制病毒的吸附或融合。通过我们的研究，我们在体外发现了许多具有抗HIV-1活性的中草药。唇形科植物中的草本植物具有很强的抗HIV-1活性，合理且易于栽培，具有非常重要的意义。寻找这些唇形科植物中的共同活性物质具有重要意义。