CARBOHYDRATE CHAIN ANALYSIS OF FORENSIC SAMPLES

法医样品的碳水化合物链分析

• 批准号: 07670485
• 负责人: TAKIZAWA Hisao
• 金额: $ 1.47万
• 依托单位: TOYAMA MEDICAL AND PHARMACEUTICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670485/
• 关键词: glycolipid; per-O-benzoil derivatives; ABO blood group; PCR; blood group gene; glycosyl transferase; transfection; 死後経過時間; 液体クロマトグラフ; 臓器特異性; 種属特異性; postmortem interval; glycolipid; HPLC; benzoyl derivatives

The members in this research group engaged themselves in the studies of ABO blood group system and carbohydrate analysis of forensic samples. Results under this research project were classified into following 3 items.(1)Neutral glycosphingolipids as their per-O-benzoyl derivatives were analyzed with high-performance liquid chromatography (HPLC). Various glycosphingolipids were found in tissue specific nature. HPLC patterns of glycosphingolipids could be introduced for the tissue identification of forensic samples.(2)ABO genotyping of forensic material was established by PCR-RFLP methods. Blood, saliva and seminal stains on the remains could be examined without trouble shooting, but some hair samples gave unreliable results.(3)Transcriptional regulatory mechanism was studied by the transfection of reporter plasmids containing various length of upstream sequence of human ABO blood group gene into a gastric cancer cell line. The just upstream sequence of the gene was the transcription start site (cap site), and the transcription could be enhanced by a CBF/NF-Y which binds the minisatellite on the further upstream sequence of the gene.

本课题组主要从事法医学标本ABO血型系统和糖类分析的研究。本研究项目的结果分为以下3项。（1）采用高效液相色谱法（HPLC）分析了中性鞘糖脂及其过氧苯甲酰衍生物。鞘糖脂的种类繁多，具有组织特异性。鞘糖脂的HPLC图谱可用于法医样品的组织鉴定。（2）应用PCR-RFLP方法对法医学材料进行ABO基因分型。尸体上的血迹、唾液和精液污渍可以毫无困难地进行检查，但一些头发样本的结果不可靠。（3）将含有不同长度人ABO血型基因上游序列的报告质粒转染胃癌细胞系，研究其转录调控机制。该基因的上游序列是转录起始位点（cap site），并且可以通过结合该基因更上游序列上的小卫星的CBF/NF-Y来增强转录。

项目成果

山口弘信: "Ampli Type PM 検出キットによる DNA多型の検出について-富山県における出現状況と唾液斑痕及び毛髪への応用-" 日法医誌. 49(3). 199-204 (1995)

Hironobu Yamaguchi：“使用 Ampli 型 PM 检测试剂盒检测 DNA 多态性 - 富山县的发生状况及其在唾液斑点和头发中的应用 -”日本法医学杂志 49（3）（1995）。

Kominato Y., Galson D.L., Waterman W.R., Webb A.C.and Auron P.E.: "Monocyte expression of the human prointerleukin 1 beta gene (IL1B) is dependent on promoter sequences which bind the hematopoietic transcription factor Spi-1/PU.1." Mol.Cell.Biol.15. 58-68

Kominato Y.、Galson D.L.、Waterman W.R.、Webb A.C. 和 Auron P.E.：“人白介素原 1 β 基因 (IL1B) 的单核细胞表达依赖于结合造血转录因子 Spi-1/PU.1 的启动子序列。”

Shimada I., Kominato Y., Hata N.and Takizawa H.: "Analysis of organ glycolipids." Jpn.J.Legal Med.52. (1999)

Shimada I.、Kominato Y.、Hata N. 和 Takizawa H.：“器官糖脂的分析”。

Kominato Y.: "Monocyte expression of the human prointerleukin 1β gene(IL1B) is dependent on promoter sequences which bind the hematopoietic transcription factor Spi-1/PU.1." Mol.Cell.Biol.15. 58-68 (1995)

Kominato Y.：“人白介素原 1β 基因 (IL1B) 的单核细胞表达依赖于结合造血转录因子 Spi-1/PU.1 的启动子序列。Mol.Cell.Biol.15 (1995)。

Yamaguchi H.: "Frequency of the three STR loci(TPOX,CSF1PO,TH01),in a Japanese population determined using a Gene Print TM STR Multiplex Kit." Jpn.J.Legal Med.50(3). 163-167 (1996)

Yamaguchi H.：“使用 Gene Print TM STR Multiplex Kit 确定日本人群中三个 STR 位点（TPOX、CSF1PO、TH01）的频率。”