Estimation of postmortem interval by changes in brain lipids : Application to estimate the time after brain death

通过脑脂质变化估计死后间隔：应用于估计脑死亡后的时间

• 批准号: 07670502
• 负责人: NIITSU Hisae
• 金额: $ 1.47万
• 依托单位: Iwate Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670502/
• 关键词: Postmortem change; Brain; Lipid; Rats

For the study of estimation of the time after death, postmortem changes in brain lipids were investigated. Brain lipids in 44 forensic autopsy cases were examined. Total lipids, total phospholipids and total free fatty acids were measured, and percentage of free fatty acid composition was calculated. Percentage of phospholipid to total lipid decreased, whereas that of total free fatty acids increased in accordance with postmortem interval. Percentages of 16 : 0 and 18 : 0 in the free fatty acid were high, although the former tended to decrease in accordance with postmortem interval.The influence of modes of killing and storage temperature on the changes of lipids was compared. Rats were killed by either nitrogen asphyxiation, decapitation, or intraperitoneal injection of sodium pentobarbital. The bodies were maintained at 15ﾟC,23ﾟC and 30ﾟC in incubator up to 120 hours. Among the free fatty acid, the composition of 18 : 0 and 20 : 4 decreased, whereas that of 22 : 6 increased with time, although the amounts of total free fatty acid of rats died of pentobarbital intoxication were somewhat lower than those of nitrogen asphyxiation and decapitation. The modes of killing did not significantly affect the changes of lipids presumably because of short duration of agonal stage inherent in them. The storage temperature of rat bodies influenced the changes of lipids, which were greater at 30ﾟC than at 15ﾟC and 23ﾟC.The influence of duration of agonal stage on the change of lipids was investigated by pentobarbital intoxication. The amounts of total free fatty acid of rats killed by decapitation after injection of pentobarbital were lower than those of rats killed by decapitation without pentobarbital injection. The amounts of total free fatty acid in rats died 15 or 30 minutes after pentobarbital injection were lower than those of 5 minutes.We intend to investigate the changes of brain lipids for estimation of the time after brain death.

为了研究死亡后时间的估计，研究了脑脂质的死后变化。本文对44例法医尸检脑组织进行了脂质检测。测量总脂质、总磷脂和总游离脂肪酸，并计算游离脂肪酸组成的百分比。随着死亡时间的延长，磷脂占总脂质的比例逐渐降低，而总游离脂肪酸的比例逐渐升高。16岁的孩子：0和18：结果表明，不同屠宰方式和不同贮藏温度对脂肪酸含量的影响不同，但随着死亡时间的延长，脂肪酸含量呈下降趋势。通过氮气窒息、断头或腹腔注射戊巴比妥钠处死大鼠。尸体在15 ℃、23 ℃和30 ℃的培养箱中保存长达120小时。在游离脂肪酸中，18：0和20：4的组成随时间的延长而减少，而22：6的组成随时间的延长而增加，但戊巴比妥中毒死亡大鼠的总游离脂肪酸含量略低于氮窒息和断头死亡大鼠。杀死方式并没有显着影响脂质的变化，可能是因为它们固有的濒死阶段持续时间短。大鼠尸体保存温度对脂质变化有影响，30 ℃时的影响大于15 ℃和23 ℃。注射戊巴比妥后断头处死大鼠的总游离脂肪酸含量低于不注射戊巴比妥断头处死大鼠。大鼠注射戊巴比妥后15分钟或30分钟死亡，脑内总游离脂肪酸含量低于注射戊巴比妥后5分钟死亡的大鼠，本实验旨在观察脑内脂质的变化，以推测脑死亡时间。

项目成果

新津 ひさえ: "脂脂質の死後変化-剖検例における脳脂質の変化-" 法医学の実際と研究. 38. 183-190 (1995)

Hisae Niitsu：“死后脂质变化 - 尸检病例中脑脂质的变化 -” 法医学实践与研究 38. 183-190 (1995)。

"Postmortem Changes of Brain lipid-Changes of Brain Lipid in Forensic Autopsy cases-" the Research and Practice in Forensic Medicine. 38. 183-190 (1995)

《死后脑脂质的变化——法医尸检案件中脑脂质的变化——》法医学研究与实践。