Study of airway reactivity in chimera mouse

除了自主神经控制外，气道反应性被认为是由气道的各种细胞决定的，如气道平滑肌细胞、上皮细胞、肺巨噬细胞、肥大细胞、嗜酸性粒细胞和淋巴细胞，它们是气道的组成部分。高气道反应性的A/J小鼠和低气道反应性的C3H/He小鼠的Flmice均表现出较低的气道反应性，提示常染色体隐性遗传。因此，研究A/J和C3H/He小鼠（A/J**C3H）嵌合体小鼠的气道反应性是非常有趣的。方法1)采用Konzett Rossler法测定小鼠气道反应性。2)嵌合体小鼠：A/J和C3H/He雌性小鼠注射人促性腺激素，与成年雄性小鼠笼合。2 d后，切除卵管和子宫，用培养液冲洗卵管并培养，收集胚胎。在8细胞期，加入植物血凝素（phytohemagglutinin， PHA）将A/J和C3H胚混合融合。将融合胚移植到假孕ICR养母的子宫内。结果3次实验只产生A/J**C3H嵌合体小鼠1只。其气道对甲胆碱的反应性介于A/J和C3H之间（分别为11.4U、10.9U和16.6U）。本研究表明嵌合体小鼠是肺移植的实验模型。

In addition to autonomic nerve control, airway reactivity is thought to be determined by various cells such as airway smooth muscle cells, epithelial cells pulmonary macrophages, mast cells, eosinophils and lymphocytes which are components of the airway. Flmice from A/J mice with higher airway responsiveness and C3H/He mice with lower responsiveness were reported to show lower airway responsiveness, indicating the autosomal recessive heredity. Therefore, it is very interesting to examine the airway reactivity of the chimera mouse of A/J and C3H/He mouse (A/J**C3H).1. Methods1) Estimation of mouse airway reactivity was carried out according to Konzett Rossler method.2) Chimera mouse : A/J and C3H/He female mice were injected ip with human gonadtropin and caged with adult males. 2 days after, ovi duct and uteri were removed and the emfroys were collected by washing ovi duct by medium and cultured. At the 8-cell stage, A/J and C3H embroys were mixed and fused by adding phytohemagglutinin (PHA). The fused embroys were transferred to the uteri of pseudpregnant ICR foster mothers.2. ResultsFrom three experiments, only one chimera mouse (A/J**C3H) was produced. Its airway reactivity to methacholine was intermediate between values of A/J and C3H (11.4U,10.9U and 16.6U,respectively).The present study showed the chimera mouse is an experimental model of the lung transplantation.