Cold activation of complement in sera from patients with chronic hepatitis C

部分慢性肝病患者血清中补体活性在低温贮藏过程中降低。这种现象被称为“补体冷激活”。测定慢性乙型肝炎和丙型肝炎患者在4 -氨基甲酸乙酯下储存过夜后血清的溶血活性。327例慢性丙型肝炎患者血清中有236例（72%）补体冷活化导致溶血活性丧失，56例（17%）血清溶血活性下降，比94例慢性乙型肝炎患者血清中的1例（1%）和13例（14%）更为频繁。67例慢性丙型肝炎患者接受淋巴母细胞油或重组干扰素- α治疗后随访6个月。治疗前补体冷活化56例（溶血活性丧失44例，溶血活性降低12例）。冷激活消失了，反应是？其中15例（85%）观察到。其余38例患者持续或波动，其中只有6例（16%）对干扰素有反应。补体的冷活化一度在干扰素完成时消失，在一些无应答者中重新出现。这些结果提示补体的冷活化可能与丙型肝炎病毒血症有关，并有助于监测慢性丙型肝炎患者对干扰素的反应。

Complement activity in sera from some patients with chronic liver disease decreases during the storage at low temperature. The phenomenon is known as "cold activation of complement".Hemolytic activity was determined in sera from patients with chronic hepatitis B and C after storage at 4ﾟC overnight. The loss of hemolytic activity by cold activation of complement was observed in 236(72%) and decrease of activity in 56(17%) out of 327 sera from patients with chronic hepatitis C,much more frequently than 1(1%) and 13(14%) of 94 sera from patients with chronic hepatitis B.Sixty-seven patients with chronic hepatitis C received lymphoblastoil or recombinant interferon-alpha and followed 6 months after treatment. Before treatment 56 patients of them showed the cold activation of complement (44 showed loss of hemolytic activity, 12 showed decrease of hemolytic activity) . The cold activation disappeared and the response was ? bserved in 15(85%) of them. It persisted or fluctuated in the remaining 38 patients, only 6(16%) of them responded to interferon. The cold activation of complement once disappeared at the completion interferon and reappered in some non-responders.These results suggested that the cold activation of complement would be associated with hepatitis C viremia, and useful in monitoring the response to interferon in patients with chronic hepatitis C.