Role of free radicals on the reperfusion injury of ischemic heart -study with radical scavengers-
自由基对缺血性心脏再灌注损伤的作用-自由基清除剂的研究-
基本信息
- 批准号:07670806
- 负责人:
- 金额:$ 1.41万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1995
- 资助国家:日本
- 起止时间:1995 至 1996
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Object :We studied the role of free radicals on the reperfusion injury in the various experimental models of ischemic heart.Results :1. We could detect the free radicals with electron spin resonance (ESR) method using spin trapping agent, POBN,in the rat and dog models of ischemia-reperfused heart from 30 sec to 5 min after reperfusion.2. Ca^<++>-ATPase activity of sarcoplasmic reticulum (SR) and its composing protein was earlier degradated in the ischemia-reperfused heart than ischemia-nonreperfused heart in the rat and dog models.3. In the perfused rat heart and isolated myocyte, free radical generations from mitochondria and endothelial cell were detected visually using Cerium chloride, reactant to the hydrogenperoxide, with ultrastructural observation.4. In the stunned heart of the dog model (15 min ischemia followed by 60 min reperfusion), contractile function and its SR function were more improved with treatment of superoxide dismutase (SOD) and ACE-inhibitor (alacepril).Conclusion :Free radial generation was one of the initial factors of the reperfusion injury.
目的:研究自由基在各种实验性心脏缺血再灌注损伤中的作用。采用自旋捕集剂POBN的电子自旋共振(ESR)方法检测大鼠和犬缺血-再灌注心脏再灌注后30 s ~ 5 min的自由基.大鼠和犬心肌缺血-再灌注模型心肌肌浆网Ca^++-ATPase活性及其组成蛋白的降解早于缺血-非再灌注模型.在灌流的大鼠心脏和离体心肌细胞中,用氯化铈作为过氧化氢的反应物,观察线粒体和内皮细胞产生自由基的情况,并进行超微结构观察.超氧化物歧化酶(SOD)和ACE抑制剂(丙氨蝶呤)能明显改善犬顿抑心脏(缺血15 min再灌注60 min)的收缩功能和SR功能。结论:自由基的产生是再灌注损伤的起始因素之一。
项目成果
期刊论文数量(12)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Katsumichi Iijima: "Dose ACE-I (alacepril) Scarvenge Free Radicals on Stunned Myocardium?" VIII Biennial Meeting International Society for Free Radical Research,Abstract book. 152-3 (1996)
Katsumichi Iijima:“给昏迷的心肌服用 ACE-I(阿拉普利)清除自由基吗?”
- DOI:
- 发表时间:
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- 影响因子:0
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- 通讯作者:
Ryuji Ueda,et al.: "Production of Hydrogen Peroxide during Hypoxia-regenerated in Isolated Myocytes." J Mol Cell Cardiol. 28. A316 (1996)
Ryuji Ueda 等人:“离体心肌细胞缺氧再生期间过氧化氢的产生”。
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- 影响因子:0
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Ryuji Ueda,et al.: "Histochemical Demonstration of Hydrogen Peroxide Generation in Reperfused Rat Heats" VIII Biennial Meeting International Society for Free Radical Research,Abstract book. 153-3 (1996)
Ryuji Ueda 等人:“再灌注大鼠发热中过氧化氢生成的组织化学演示”第八届国际自由基研究学会双年会,摘要书。
- DOI:
- 发表时间:
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- 影响因子:0
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- 通讯作者:
Ryuji Ueda,et al.: "Histochemical Demonstration of Hydrogen Peroxide Generation in Reperfused Rat Hearts" VIII Biennial Meeting International Society for Free Radical Research,Abstract book. 153-3 (1996)
Ryuji Ueda 等人:“再灌注大鼠心脏中过氧化氢生成的组织化学演示”第八届国际自由基研究学会双年会,摘要书。
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- 影响因子:0
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岩田俊樹: "心筋再潅流障害にSODは有効が?-内因性catalaseの意義-" Japanese Circuration Journal. 59. 394 (1995)
Toshiki Iwata:“SOD 对心肌再灌注损伤有效吗?-内源性过氧化氢酶的意义-”日本循环杂志 59. 394 (1995)。
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KATAGIRI Takashi其他文献
KATAGIRI Takashi的其他文献
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{{ truncateString('KATAGIRI Takashi', 18)}}的其他基金
Development Principle of Imageguide for Raman Spectroscopy by Hollow Light Guiding
空心光导拉曼光谱像导的研制原理
- 批准号:
23760297 - 财政年份:2011
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Young Scientists (B)
Immunohistochemical and molecular biological studies of vascular smooth muscle cell proliferation after coronary stent implantation
冠状动脉支架植入后血管平滑肌细胞增殖的免疫组织化学和分子生物学研究
- 批准号:
11670708 - 财政年份:1999
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Mechanisms of Severe Pump Failure in Acute Myocardial Infarction and Alteration of Cardiac Metabolisms.
急性心肌梗塞严重泵衰竭的机制和心脏代谢的改变。
- 批准号:
01570503 - 财政年份:1989
- 资助金额:
$ 1.41万 - 项目类别:
Grant-in-Aid for General Scientific Research (C)
相似海外基金
NOVEL MECHANISMS OF MITOCHONDRIAL FREE RADIAL GENERATION
线粒体自由基产生的新机制
- 批准号:
6480123 - 财政年份:2002
- 资助金额:
$ 1.41万 - 项目类别:
NOVEL MECHANISMS OF MITOCHONDRIAL FREE RADIAL GENERATION
线粒体自由基产生的新机制
- 批准号:
6625924 - 财政年份:2002
- 资助金额:
$ 1.41万 - 项目类别: