Gut, Immune organ-mucosal immunity of gastro-intestinal tract-

肠道、免疫器官-胃肠道粘膜免疫-

• 批准号: 07670867
• 负责人: ODA Megumi
• 金额: $ 1.47万
• 依托单位: Okayama University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670867/
• 关键词: Immune organ; Guit epithelial cell; chemokine; IL8; Ussing chamber; Mast cell; Ussing chamber; IL-8; MIP-1α; MCP-1; MCAF; RANTES

Background and Objective : To clarify a role of the gastrointestinal tract in mediating immunity, we examined following three points between 1995 and 1996, using experiments where gastrointestinal epithelial cells are infected by bacteria and viruses. (1) Whether mRNA of the chemokine family is expressed in epithelial cells, (2) whether the chemokine mRNAs are translated into proteins and (3) whether the chemokine proteins have any functions. As a result, the patterns of chemokines produced by gastrointestinal epithelial cells due to bacterial or viral infections differed from each other, which was thought to be the main cause of the diversity in local mobilization of inflammatory cells and inflammatory responses against various infections. Furthermore, IL-8 obtained from supernatant of cell culture was concentrated, then added to neutrophils and briefly cultured. Subsequently, expression levels of CD11b/18 and Leu8 (L-selectin) on the surface of neutrophils were measured by FACS,resul … More ting in an increase in CD11b/18 and a decrease in L-selection expression. And anti-IL-8 antibody partially inhibited these increases and decreases, suggesting that IL-8 in supernatant of cell culture plays a role in activating neutrophils. However, other cytokines are also involved.Then in 1997, we used an Ussing chamber to electrophysiologically analyze the influence of various chemokines and supernatant of cell culture on T84 and/or 18CO (human gastrointestinal fibroblasts) and/or neutrophils coculture systems. In addition, establishment of a mast cell line was attempted, to examine the effects of mast cells instead of neutrophils.Methods and Results : Coculture of gastrointestinal epithelial cells with fibroblasts facilitated membrane depolarization, and coculture with neutrophils further facilitated depolarization. A mast cell line was established using a culture system where IL-6, stem cell factor and prostaglandin E_2 were added to mononuclear cells from cord blood. We intend to further investigate the coculture system with this cell lone. Less

背景和目的：为了阐明胃肠道在介导免疫中的作用，我们在1995年至1996年间通过胃肠道上皮细胞被细菌和病毒感染的实验检查了以下三点。 (1)趋化因子家族的mRNA是否在上皮细胞中表达，(2)趋化因子mRNA是否翻译成蛋白质，(3)趋化因子蛋白质是否具有任何功能。因此，细菌或病毒感染引起的胃肠道上皮细胞产生的趋化因子模式各不相同，这被认为是炎症细胞局部动员和针对各种感染的炎症反应多样性的主要原因。此外，将从细胞培养上清液中获得的IL-8浓缩，然后添加到嗜中性粒细胞中并短暂培养。随后，通过 FACS 测量中性粒细胞表面 CD11b/18 和 Leu8（L-选择素）的表达水平，结果发现 CD11b/18 增加，L-选择表达减少。而抗IL-8抗体部分抑制了这些增加和减少，表明细胞培养物上清液中的IL-8在激活中性粒细胞中发挥作用。然而，其他细胞因子也参与其中。然后在1997年，我们使用Ussing小室从电生理学角度分析了各种趋化因子和细胞培养上清液对T84和/或18CO（人胃肠道成纤维细胞）和/或中性粒细胞共培养系统的影响。此外，还尝试建立肥大细胞系，以检测肥大细胞代替中性粒细胞的作用。​​方法和结果：胃肠道上皮细胞与成纤维细胞共培养促进膜去极化，与中性粒细胞共培养进一步促进去极化。使用将IL-6、干细胞因子和前列腺素E_2添加至来自脐带血的单核细胞的培养系统建立肥大细胞系。我们打算进一步研究与该细胞的共培养系统。较少的

项目成果

Megumi ODA: "Modulation of Epithelial Ion Secretion By Nentrophils and Fibroblasts" Clinical Immunology and Immunopathology. 76・1. S41-S41 (1995)

Megumi ODA：“嗜中性粒细胞和成纤维细胞对上皮离子分泌的调节”临床免疫学和免疫病理学76·1（1995）。

Megumi Oda: "Comparison of Viral vetsus Bacterial - Inducecl Production of Chemokines In Gut Epithelial Cells" Clinical Immunology and Immunopathology. 76・1. S42-S42 (1995)

Megumi Oda：“病毒细菌的比较 - 肠道上皮细胞中趋化因子的产生”临床免疫学和免疫病理学 76・1（1995）。

Megumi ODA: "Modnlation of Epithelial Ion Secretion By Nentrophils and Fibroblasts" Clinical Immunology and Immunopathology. 76・1. S41-S41 (1995)

Megumi ODA：“中性粒细胞和成纤维细胞对上皮离子分泌的调节”临床免疫学和免疫病理学76·1（1995）。