Fundermental study of lysozyme crystallization at high pressure

溶菌酶高压结晶的基础研究

• 批准号: 07804036
• 负责人: SAWAMURA Seiji
• 金额: $ 1.41万
• 依托单位: RITSUMEIKAN UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07804036/
• 关键词: high pressure; lysozyme; crystallization; solubility; 圧力; 結晶

First our high pressure system for the solubility measurement was checked up to 300 MPa using ammonium chloride and cesium chloride as solutes. The reproducibility of the solubility was (]SY+-[)0.5%. On the other hand, conditions of the solubility measurement of lysozyme were surveyed at 0.1 MPa. and get following results. (1) The solid state of lysozyme in the solubility measurement depends on the initial concentration of the solute. It means that sample solution of lysozyme should be prepared at low concentration. (2) Shaking time to attain to the saturation is very long. It takes several weeks though those for CsCl and NH4Cl are at most one or two days.Next the solubility was checked at high pressure up to 200 MPa. It is found that (1) rate of the precipitaion decreased with increasing pressure and (2) the solubility increased, i.e., 7 mg/ml at 0.1 MPa to 20 mg/ml at 200 MPa.

首先，使用氯化铵和氯化铯作为溶质，对我们的高压溶解度测量系统进行了测试，测试压力高达300兆帕。溶解度的重现性为(SY+-)0.5%。另一方面，考察了0.1 Mpa下测定溶菌酶溶解度的条件。并得到以下结果。(1)溶菌酶在溶解度测定中的固体状态取决于溶质的初始浓度。这意味着溶菌酶的样品溶液应在较低浓度下制备。(2)达到饱和的摇床时间很长。氯化亚锡和氯化铵的溶解度最多一到两天，但需要几个星期的时间。然后在200兆帕的高压下检查溶解度。结果表明：(1)沉淀率随压力增加而降低；(2)溶解度增加，0.1 Mpa时为7 mg/ml，200 Mpa时为20 mg/m l。

项目成果

T.Nakai, S.Sawamura, Y.Taniguchi, and Y.Yamaura: "Effect of Pressure on the Viscosity B Coefficient of Cesium Chloride in water" Mater.Sci.Res.Int.2. 143-147 (1996)

T.Nakai、S.Sawamura、Y.Taniguchi 和 Y.Yamaura：“压力对水中氯化铯粘度 B 系数的影响”Mater.Sci.Res.Int.2。

中井武裕: "298.2Kにおけるハロゲン化アルカリ水溶液の高圧粘性挙動" 材料. 45. 280-285 (1996)

Takehiro Nakai：“碱金属卤化物水溶液在 298.2K 时的高压粘性行为”材料 45. 280-285 (1996)。

巻本彰一: "ズブチリシンBPNの熱および圧力変性機構" 材料. 45. 247-279 (1996)

Shoichi Makimoto：“枯草杆菌蛋白酶 BPN 的热压变性机制”材料 45. 247-279 (1996)。

瀬戸倫義: "NMR Studies an Dynamic Structure of Hydrated Water Molecnles in Alcohol-D_2O Solutions" J. Mol. Liquids. 70. 1-9 (1996)

Michiyoshi Seto：“NMR 研究酒精-D_2O 溶液中水合水分子的动态结构”J. Mol Liquids。

谷口吉弘: "Catalysts,Polymeric (Pressure Effects),Polymeric Material Encyclopedia" CRC Press Inc. Vol.2, 999-1004 (1996)

Yoshihiro Taniguchi：“催化剂，聚合物（压力效应），聚合物材料百科全书”CRC Press Inc. Vol.2, 999-1004 (1996)