Approach to identify cellular factor(s) which determine spieces-and tissue-specificity of HTLV-infection

我们已经开发了一种系统，通过该系统，携带GFP的HIV基因组经由HTLV-I包膜被转导到靶细胞的细胞核中（Koito et al.，手稿正在准备中）。除了常规的GFP之外，携带在395 nm（紫色）激发的突变GFP的HIV载体正在构建中。使用这些系统，将阐明HTLV-I感染的靶细胞群。用鼠逆转录病毒载体从人骨肉瘤（HOS）细胞中构建了用于分离HTLV-Ⅰ受体基因的cDNA文库。通过将cDNA文库转染到包装BOSC细胞系中产生的高滴度重组逆转录病毒（Kitamura等人，Proc.Natl.Acad.Sci.USA 92.1995）接种到显示对HTLV-1感染具有抗性的小鼠NIH 3 T3细胞中。该方法将用于鉴定与HTLV-I感染有关的因素。

We have developed the system by which HIV genome carrying GFP is transduced into nucleus of target cells via through HTLV-I envelope (Koito et al., manuscript in preparation). In addition to conventional GFP, HIV vector carrying mutated GFP which excites at 395 nm (violet) is under construction. Using these systems, the target cell population to HTLV-I infection will be clarified. The cDNA library for the isolation of the gene coding for the HTLV-I receptor has been constructed from the human osteosarcoma (HOS) cells by using murine retrovirus vector. High titer recombinant retrovirus generated by transfection of cDNA library into packaging BOSC cell line (Kitamura et al., Proc.Natl.Acad.Sci.USA 92.1995) will be inoculated into the mouse NIH3T3 cells which is shown to be resistant to HTLV-I infection. This approach will be employed to identify the factor (s) involved in HTLV-I infection.