hepatic is chemia on and reper Protectiue effect anti-adhesion molecules antibody ies on hepatic is chemia and reperfusion iniury, and its clinical application.

我们研究了粘附分子单克隆抗体（mAb）对肝缺血再灌注损伤、肝脏保存和内毒素休克诱导的肝损伤的作用和库普弗细胞功能的调节。虽然肝脏缺血90分钟不能使动物存活，但对ICAM-1和LFA-1进行单抗预处理使存活率提高到57%。肝脏中性粒细胞数量在缺血90 min再灌注后24小时内持续增加。单抗预处理能抑制中性粒细胞的浸润和过氧化脂质的升高，促进再灌注后肝脏ATP的恢复。在内毒素休克肝损伤模型实验中也观察到类似的保护作用。在大鼠肝移植实验中，一种含有甲磺酸那莫他酯和库普弗细胞阻断剂的新型冲洗液发挥协同作用，提高了冷保存肝移植后的存活率。提示Kupffer细胞和中性粒细胞参与了肝脏缺血再灌注损伤和内毒素休克诱导的肝损伤，对粘附分子进行单抗预处理有助于预防缺血性肝细胞损伤和内毒素休克肝细胞损伤。

We investigated the efficacy of the monoclonal antibodies (mAb) to adhesion molecules and the modulation of Kupffer cell function on hepatic ischemia-reperfusion injury, liver preservation and endotoxin shock induced hepatic injury. Although ischemia of the liver for 90 min did not permit survival of the animals, pretreatment with mAb to ICAM-1 and LFA-1 increased the survival rate to 57%. The number of neutrophils in the liver increased continuously up to 24 hours after reperfusion following 90 min ischemia. Pretreatment with mAb suppressed the infiltration of neutrophils and the elevation of lipid peroxide, and enhanced the recovery of hepatic ATP after reperfusion. A similar protective results we also observed in the model experiment of endotoxin shock induced liver injury. In the experiment of rat liver transplantation, a new rinse solution containing nafamostat mesilate and Kupffer cell blockade exert synergistic effects, leading to increased survival after cold-preserved liver transplantation. These results suggest that Kupffer cell and neutrophils contribute to ischemia-reperfusion injury in the liver and endotoxin shocked induced liver injury, and the pretreatment with mAb to adhesion molecules are useful for prevention of ischemic liver cell injury and endotoxin shocked liver cell injury.