Immunohistochemical studies of abnormal extrinsic nerve fibers in Hirschsprung's disease

先天性巨结肠异常外源神经纤维的免疫组织化学研究

• 批准号: 07807163
• 负责人: NIRASAWA Yuji
• 金额: $ 1.02万
• 依托单位: Kyorin University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07807163/
• 关键词: HPC-1 antigen; Hirschsprung's disease; exocytosis-related protein; 無神経節部腸管

The HPC-1 antigen was originally identified as a neuron-specific membrane protein in the central nervous system. So, as the first step of the experiments, the presence of HPC-1 antigen in the nervous system of the rat gastrointestinal tract was immunohistochemically confirmed. The HPC-1 is thought to suppress the excess axon-collateral sprouting.Abnormal extrinsic nerve fibers in Hirschsprung's disease was then immunohistochemically examined using antibody againt the exocytosis-related protein which the HPC-1belongs to. All six exocytosis-related Proteins were succesfully demonstrated in normal sigmoid colon. On the other hand, the HPC-1 was not detected in aganglionic portion of the colon, which may explain the reason of exess extrins nerve fibers in aganglionic portion of the colon in Hirschsprung's disease.

HPC-1抗原最初被鉴定为中枢神经系统中的神经元特异性膜蛋白。因此，作为实验的第一步，通过化学方法证实大鼠胃肠道神经系统中存在HPC-1抗原。HPC-1被认为是抑制过度的轴突侧支发芽的，然后用抗HPC-1所属的胞吐相关蛋白的抗体对先天性巨结肠病中异常的外源性神经纤维进行免疫组化检查。所有六种胞吐相关蛋白均在正常乙状结肠中成功表达。而HPC-1在无神经节段结肠未见表达，这可能是先天性巨结肠无神经节段肠外膜神经纤维增多的原因。

项目成果

Nirasawa Y,Ito Y,Seki N,Akagawa K.: "HPC-1/syntaxin-1A activity in the enteric nervous system of developing rat gastrointestinal tract." Journal of Smmoth Muscle Reseach. 33-2. 61-66 (1997)

Nirasawa Y、Ito Y、Seki N、Akakawa K.：“发育中的大鼠胃肠道的肠神经系统中的 HPC-1/syntaxin-1A 活性。”

Nirasawa Y: "HPC-1/Syntoxin-1A actiung in the entetic nervous system of developing rat gastrointcstinal tract" Jorrnal of Smooth Muscle Research. 33-2. 61-66 (1997)

Nirasawa Y：“HPC-1/Syntoxin-1A 在发育中的大鼠胃肠道的肠神经系统中发挥作用”平滑肌研究杂志。

Y.Nirasawa: "HPC-1/Syntaxin-1A activity in the entcric nervens system of developing rat gastrointestinal tract" Journal of Smooth Muscle Research. 33-2. 61-66 (1997)

Y.Nirasawa：“发育中的大鼠胃肠道内神经系统中的 HPC-1/Syntaxin-1A 活性”平滑肌研究杂志。