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Effects of matification on immunity in hosts prohibited from oral alimentation

成熟化对禁止口服食物的宿主免疫力的影响

基本信息

批准号：
07838037
负责人：
ABE Shigeru
金额：
$ 1.41万
依托单位：
Teikyo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07838037/
关键词：
Parenteral alimentation IVH Neutrophils Candida albicans Mastification Saliva Lactoferrin Lysozyme Candida

项目摘要

Parenteral alimentation is clinically applied to patients with intestinal dysfunction who are prohibited from oral alimentation. Their immunological status is often greatly suppressed making them vulnerable to opportunistic fungal infection. In this study we focused on antifungal activity (growth inhibition activity against fungi) of neutrophils in hosts unable to take oral alimentation. Neutrophils obtained from peripheral blood of normal individuals clearly inhibited the growth of Candida albicans a in vitro.. In some patients with parenteral alimentation, the anti-Candida activity of neutrophils appeared to be suppressed, but variations in the activities among patients with different diseases made clear observation impossible. The effects of a glucose and amino acid mixture prescribed for parenteral alimentation on ant-Candida activity of neutrophils were then examined. More than 1.0% of glucose inhibited the anti-Candida activity of human neutrophils in a dose-dependent manner. Thi … More s glucose effect was reduced by the addition of the amino acid mixture (especially cystein solution) which is clinically prescribed with a carbohydrate mixture solution in Japan. These results suggest that the amino acid mixture may act as a neutralizre of the suppressive action of glucose for anti-Candida activity of neutrophils.The direct effect of mastification on the immunity of mice prohibited oral alimentation could not be determined, since parenteral alimentation of these animals is very difficult. We examined instead the effects of components of saliva, the secretion of which is a main physiological response induced by mastification. A saliva component.lactoferrin augmented anti-Candaida activity of murine neutrophils. We observed that not only lactoferrin but also lysozyme inhibited the Candida growth in vitro synergistically with antifungal azoles. Oral administration of lactoferrin protected mice from lethal systemic infection by C.albicans. These findings suggest that mastification may augment the host-defense mechanisms against Candida infection perhaps through enhancement of secretion of saliva. Less

肠外营养在临床上应用于肠道功能障碍而不能口服营养的患者。他们的免疫状态往往受到极大的抑制，使他们容易受到机会性真菌感染。在这项研究中，我们专注于抗真菌活性（对真菌的生长抑制活性）的中性粒细胞在主机无法采取口服营养。从正常人外周血中获得的中性粒细胞在体外明显抑制白色念珠菌a的生长。在一些胃肠外营养的患者中，中性粒细胞的抗念珠菌活性似乎受到抑制，但不同疾病患者的活性差异使得无法进行明确的观察。然后检查了用于肠外营养的葡萄糖和氨基酸混合物对中性粒细胞抗念珠菌活性的影响。超过1.0%的葡萄糖以剂量依赖方式抑制人中性粒细胞的抗念珠菌活性。Thi ...更多信息的葡萄糖效应通过添加氨基酸混合物（特别是半胱氨酸溶液）而降低，该氨基酸混合物在日本临床上与碳水化合物混合物溶液一起使用。这些结果表明，氨基酸混合物可能作为中性粒细胞的抗念珠菌活性的葡萄糖抑制作用的中和剂。由于这些动物的胃肠外营养是非常困难的，因此，不能确定咀嚼对禁止口服营养的小鼠的免疫的直接影响。相反，我们研究了唾液成分的影响，唾液的分泌是由咀嚼引起的主要生理反应。一种唾液成分。乳铁蛋白增强鼠中性粒细胞的抗Candaida活性。我们观察到，不仅乳铁蛋白，而且溶菌酶抑制念珠菌生长在体外协同抗真菌唑类。口服乳铁蛋白保护小鼠免受致命的全身感染白色念珠菌。这些发现表明，咀嚼可能通过增加唾液分泌来增强宿主对念珠菌感染的防御机制。少