Analysis of Anti-Carbonic Anhydrase Antibody

首先，采用免疫印迹和酶联免疫吸附试验(ELISA)方法，对风湿性自身免疫病(RAD)患者血清中抗碳水解酶(CA)抗体进行了检测。在20-30%的RAD患者血清中发现抗CA抗体，所有抗CA血清均与人CAI和CAII反应，并探讨其与临床证候的关系。其次，用合成肽分析RAD血清或健康人天然自身抗体识别的CA1和CAII抗原表位。RAD患者抗CA抗体可识别CA氨基末端的α螺旋部分和CA酶的活性区域。这可能证明了前面讨论的抗CA抗体对CA活性的抑制作用。相反，天然自身抗体仅与CA羧基末端的一部分发生反应。这些观察结果可能提示了RAD自身免疫的不同机制。我们还报告了一例具有类似CA缺乏症症状的抗CA抗体患者。

At first, using immunoblot and ELISA,the incidence of anti-carbonic anlydrase (CA) antibodies in Rheumatic autoimmune disease (RAD) was investigated. Anti-CA antibodies were found in 20-30% of sera from patients with RAD.All anti-CA sera were reactive with both human CAI and CAII.The correlation of it with clinical syndrome has been investigated. Second, epitopes on CAI and CAII antigen which recognized by RAD sera or natural autoantibody found in healthy individuals were analyzed using synthesized peptides. Anti-CA antibodies in RAD patients recognized alfa-helical portion on amino-terminus of CA and active domains of CA enzyme. This may evident the inhibitory effect of anti-CA antibodies against CA activity which previously discussed. In contrast, natural autoantibodies exclusively reacted with a portion located at carboxy-terminus of CA.These observation may suggest the distinguished mechanism of autoimmunity in RAD.We also reported a patient with anti-CA antibody who has similar symptoms to CA deficiency.