Study of metastasis-related genes in esophageal carcinoma

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1.Study of matrix metalloproteinase 7 (MMP7)1) We performed in vitro invasion assay using squamous carcinoma cell line KY150 and its transfectant with MMP cDNA.The expression of MMP7 in parent KY150 cell was very weak. On the other hand, its expression in transfectant was increasing and determined by Northern blot analysis. The invasion assay demonstrated that the invading cells were much frequently seen in transfectants than in parent cells (56% vs 10%).2) To determine the effect of anti-MMP7 antibody, we added the antibody into the culture medium. Consequently the invading ability of KY150 transfectants was dramatically inhibited by the antibody (56%*16%).These findings support the hypothesis that MMP7 is one of the major proteinases which influence the invading ability of esophageal squamous cell carcinomas.2.Study of integrin alpha6The expression of integrin alpha6 was dramatically increased in tumor tissues rather than normal tissues of the esophagus. This was determined by Northern blot analysis and immunohistochemical analysis. The tumors showing high degree of integrin alpha6 expression were those having deeper invasion and worse prognosis. we are studying the expression level of integrin beta1 and beta4. We are also studying the correlation between integrin expression and matrix metalloproteinase (MMP7) expression because some kind of MMPs may be regulated by signal transduction relating to the integrin.