INTERPOSITION NERVE GRAFTS TO RESTORE POSTOPERATIVE URINARY AND SEXUAL FUNCTIONS IN RATS.

目的直肠癌的手术治疗较早发现，考虑到术后功能维持的盆腔自主神经保留手术是推荐的，但目前存在晚期病例考虑到癌症的治愈性而不得不放弃保留手术的情况。本研究为阐明应用自体神经移植物维持膀胱癌术后功能的新术式开发的合理性进行了基础性研究，方法与结果（1）移植组在膀胱壁应用破伤风毒素C片段（transsynaptic retrograde transporter），以证实移植神经再生。2或3周后，通过移植和再生的神经，用破伤风毒素C片段逆行染色下脊髓（S2-4）的副交感神经细胞。(2)术后2 ~ 3周膀胱功能未见恢复。(3)术后5 ~ 6周，膀胱功能恢复正常，与膀胱功能恢复基本一致，提示间置神经移植物自体移植对晚期直肠癌患者术后功能恢复有重要意义。

AimBy surgery treatment of cancer of the rectum discovered early comparatively, the autonomous nerve preservation operation in the pelvis which considered function maintenance after operation is recommended, but there is the case that curability of cancer is thought about in the advanced case, and cannot but abandon preservation operation currently. This study is a fundamental study to make clear the propriety of new operation method development letting a function after advaned cancer operation maintains by using an interposition nerve grafts auto-transplantation.Methods and Results(1) In the graft group, tetanus toxin C fragment (transsynaptic retrograde transporter) was apllied to the bladder wall with a purpose to confirm the regeneration which was occured via the grafted nerve. After 2 or 3 weeks, parasympathetic nerve cells in lower spinal cord (S2-4) were retrogradely stained by tetanus toxin C fragment via the transplanted and reproducted nerve.(2) After 2 or 3 weeks, the recovered bladder function wasn't recognized.(3) After 5 or 6 weeks, the function recovery preservation was recognized and was almost near by bladder function in normality.SummaryI was able to suggest the possibility that an interposition nerve grafts auto-transplantation was extremely useful for function recovery after operation in advanced rectal cancer patients.