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ESTABL ISHMENT OF DIELECTRIC SPECTROSCOPY FOR INSTANTANEOUS EVALUATING MYOCARDIAL VIABILITY

建立瞬时评估心肌活力的介电能谱

基本信息

批准号：
07671458
负责人：
MURAKAWA Shinji
金额：
$ 1.47万
依托单位：
GIFU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

The aim of this work is to establish the dielectric spectroscopy for instantaneous evaluating myocardial viability. Canine hearts were immersed in 4ﾟC saline solution (group S) and 4ﾟC UW solution (group U) for 8 hours. We measured the peak value of tan delta loss tangent (tan delta m) of myocardium intermittently. In addition, changes of tan delta were compared with changes of myocardial ATP measured every 2 hours. During preservation the change of tan delta m (%tan delta m) correlated well with the change of ATP (%ATP) [group S : r^2=0.81, group U : r^2=0.66].And we examined whether tan delta couldpredict ventricular contractility after reperfusion. Preserved porcine hearts were immersed in 4ﾟC saline solution, and tan delta were measured intermittently. Then heterotopic cardiac transplantation followed. During preservation (150 to 360 minutes), the peak value of tan delta (tan delta m) decreased gradually from 5.5(]SY.+-。[)0.5 to 3.8(]SY.+-。[)0.8.Two hours after reperfusion, the tan delta m at the end of preservation was correlated with the percent recovery of left ventricular Emax (%Emax) (r=0.85), while preservation time was not correlated with it (r=0.37). And, changes of tan delta during preservation were compared with myocardial structural changes observed by electron microscopy. The change of tan delta m correlated with the destruction of cellular membrane structure.Dielectric spectroscopy of myocardium can reflect ischemic injury of cell during preservation. The tan delta m may be useful parameter for evaluation of myocardial viability instantaneously and prediction of the ventricular contractility after reperfusion.

这项工作的目的是建立用于瞬时评估心肌活力的介电谱。将犬心脏浸入4℃盐水溶液（S组）和4℃UW溶液（U组）中8小时。我们间歇性地测量心肌的tanδ损耗角正切(tanδm)的峰值。此外，将tanδ的变化与每2小时测量的心肌ATP的变化进行比较。在保存过程中，tanδm（％tanδm）的变化与ATP（％ATP）的变化密切相关[S组：r^2=0.81，U组：r^2=0.66]。并且我们检查了tanδ是否可以预测再灌注后的心室收缩力。将保存的猪心脏浸入4℃盐水溶液中，间歇测量tanδ。随后进行了异位心脏移植。保存期间（150~360分钟），tanδ（tanδm）峰值从5.5(]SY.+-。[)0.5逐渐下降至3.8(]SY.+-。[)0.8。再灌注后2小时，保存结束时的tanδm与左心室Emax恢复百分比（%Emax）相关（r=0.85），保存时时间与其不相关（r=0.37）。并且，将保存过程中tanδ的变化与电子显微镜观察到的心肌结构变化进行比较。 tanδm的变化与细胞膜结构的破坏有关。心肌的介电谱可以反映保存过程中细胞的缺血损伤情况。 tan δ m 可能是用于即时评估心肌活力和预测再灌注后心室收缩力的有用参数。