Identification of a differentiation-related gene in the rat central nervous system using differential display method
差异显示法鉴定大鼠中枢神经系统分化相关基因
基本信息
- 批准号:07671528
- 负责人:
- 金额:$ 1.47万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1995
- 资助国家:日本
- 起止时间:1995 至 1996
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
During development of the central nervous system (CNS), a large number of genes are expressed and interact with each other. Although the functions of certain genes involved in CNS development have been described, the process of development and differentiation in the CNS is not clearly understood. We have used the differential display method to identify genes which are differentially expressed during CNS development. Using this approach, we have identified a novel rat gene that codes for a protein of 42 kDa (RNB6) and is expressed in the developing embryonic rat brain. The level of expression of RNB6 peaks on postnatal day 1 in brain tissue and gradually decrease thereafter. The predicted amino acid sequence of RNB6 shares a 45% homology with the human actin-associated protein, vasodilator-stimulated phosphoprotein (VASP) and RNB6 contains a GlyProProProProPro (GP_5) motif, that is highly conserved feature of VASP family members. VASP functions through its GP_5 motifs as a ligand for profilin, a signal transducer for actin filament assembly and therefore is thought to control cellular motility through profilin-mediated microfilament assembly. The shared homology and common GP_5 motif of RNB6 and VASP suggest that RNB6 may take part in cellular motility during development of the CNS.
在中枢神经系统(CNS)的发育过程中,大量基因表达并相互作用。虽然已经描述了参与CNS发育的某些基因的功能,但是CNS的发育和分化过程还不清楚。我们已经使用差异显示方法,以确定在中枢神经系统发育过程中差异表达的基因。使用这种方法,我们已经确定了一种新的大鼠基因,编码42 kDa的蛋白质(RNB 6),并在发育中的胚胎大鼠大脑中表达。出生后第1天,RNB 6在脑组织中的表达水平达到峰值,此后逐渐下降。RNB 6的氨基酸序列与人肌动蛋白相关蛋白血管舒张刺激磷蛋白(vasodilator-stimulated phosphoprotein,VASP)的氨基酸序列有45%的同源性,并且RNB 6含有一个GlyProProProPro(GP_5)基序,这是VASP家族成员的高度保守特征。VASP通过其GP_5基序作为profilin的配体发挥功能,profilin是肌动蛋白微丝组装的信号转导器,因此被认为通过profilin介导的微丝组装来控制细胞运动。RNB 6和VASP具有相同的同源性和GP_5基序,提示RNB 6可能参与中枢神经系统发育过程中的细胞运动。
项目成果
期刊论文数量(9)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Yazaki T.et al.: "Inhibition of angiogenesis and growth of human non-malignant and malignant meningioma by TNP-470" J.Neuro-oncology. vol.23. 23-29 (1995)
Yazaki T.et al.:“TNP-470 对人类非恶性和恶性脑膜瘤的血管生成和生长的抑制”J.Neuro-oncology。
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- 影响因子:0
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Yazaki,T.: "Inhibition of angiogenesis and growth of human non-malignant and malignant meningiomas by TNP-470." J Neuro-oncol.23. 23-29 (1995)
Yazaki,T.:“TNP-470 抑制人类非恶性和恶性脑膜瘤的血管生成和生长。”
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- 影响因子:0
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峯田寿裕: "組み換え型単純ヘルペスウイルス1型による悪性脳腫瘍の実験的治療。" 脳神経外科. 23. 285-292 (1995)
Toshihiro Mineta:“用重组单纯疱疹病毒 1 型进行恶性脑肿瘤的实验治疗”,《神经外科》23。285-292。
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Yazaki,T.: "Inhibtion of angiogenesis and growth of human nonnalignant and nalignant meningl oma by TNP-410" J.Neuro-oncology. 23. 23-29 (1995)
Yazaki,T.:“TNP-410 对人类非恶性和恶性脑膜瘤的血管生成和生长的抑制”J.Neuro-oncology。
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- 影响因子:0
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Mineta,T.: "Attenuated multi-mutated herpes simplex virus-1 for the treatment of malignant gliomas." Nuture Medicine. 1. 938-943 (1995)
Mineta,T.:“用于治疗恶性神经胶质瘤的减毒多突变单纯疱疹病毒-1。”
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MINETA Toshihiro其他文献
MINETA Toshihiro的其他文献
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{{ truncateString('MINETA Toshihiro', 18)}}的其他基金
Analysis of glioblastoma stem cell derived from bone marrow stem cell using high-resolution array-based comparative genomic hybridization
使用基于高分辨率阵列的比较基因组杂交分析源自骨髓干细胞的胶质母细胞瘤干细胞
- 批准号:
17390403 - 财政年份:2005
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Detection of viral DNA sequences in human neuro-epithelial tumors
人类神经上皮肿瘤中病毒 DNA 序列的检测
- 批准号:
15591532 - 财政年份:2003
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Identification of a differentiation-related gene in the central nervous system using differential display method
利用差异显示法鉴定中枢神经系统分化相关基因
- 批准号:
09671429 - 财政年份:1997
- 资助金额:
$ 1.47万 - 项目类别:
Grant-in-Aid for Scientific Research (C)