Effect of donor specific antigen on graft survival

供体特异性抗原对移植物存活的影响

• 批准号: 07671752
• 负责人: MURAI Masaru
• 金额: $ 1.34万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671752/
• 关键词: renal transplantation; immunosuppression; lymphocyte subsets; DST; 免疫抑制療法

Donor-specific transfusion (DST) had once been widely accepted to improve living-related renal allograft survival. Previous studies have reported that transfusion can induce suppressor cell and/or anti-idiotypic antibodies. The aim of this study was to clarify the mechanism of effect of DST on renal allograft survival.The prospective kidney recipient received transfusion of the respective donors whole blood of 100ml at 1,8 and 15 days after its storage. During DST,patients received cyclosporine to minimize sensitization. Peripheral blood lymphocytes were analyzed by flowcytometer for the presence of surface antigenes using monoclonal antibodies.The percentage of CD8^+ cells rose significantly 1 week after the initial DST and was sustained throughout the remainder of the study, leading to a decreased CD4^+/CD8^+ cells ratio. The percentage of HLA-DR^+ cells also increased after DST.These results differ partly form those of Lenhard et al who studied the effect of blood transfusion on suppressor cells in hemodialysis patients. They showed an increased percentage of HLA-DR^+ cells as we did, but a decrease in OKT4/OKT8 ratio, concluding that monocytes act as suppressor cells in the immediate posttransfusion period.Increase in CD11b^+/CD8^+ cells in addition to increase in CD8^+ cells this study suggests participation of suppressor T cell in immunological effects of DST combined with CyA.To define whether or not DST combined with CyA still maintains immunologically beneficial effects, the MLR suppressor cell assay was performed, in which three of six patients were positive.The present study is preliminary because of the restricted number of patients studied. These results, however, suggest that DST combined with CyA can still induce suppressor cells while minimizing the sensitization.

供者特异性输血（DST）曾经被广泛接受用于改善与活体相关的同种异体肾移植物的存活率。先前的研究报道，输血可以诱导抑制细胞和/或抗独特型抗体。本研究的目的是阐明DST对肾同种异体移植物存活的影响机制。预期肾接受者在储存后1,8和15天接受各供体全血100ml的输注。在药敏试验期间，患者接受环孢素治疗以尽量减少过敏。使用单克隆抗体通过流式细胞仪分析外周血淋巴细胞是否存在表面抗原。在初始 DST 后 1 周，CD8^+ 细胞的百分比显着上升，并在整个研究的剩余时间中持续存在，导致 CD4^+/CD8^+ 细胞比率下降。 DST 后 HLA-DR^+ 细胞的百分比也增加。这些结果与 Lenhard 等人研究输血对血液透析患者抑制细胞的影响的结果部分不同。他们显示 HLA-DR^+ 细胞的百分比与我们一样增加，但 OKT4/OKT8 比率下降，得出结论单核细胞在输血后立即充当抑制细胞。除了 CD8^+ 细胞增加之外，CD11b^+/CD8^+ 细胞也增加，这项研究表明抑制性 T 细胞参与了 DST 与 CyA 联合的免疫效应。 DST联合CyA仍保持免疫学有益效果，进行了MLR抑制细胞测定，其中六名患者中的三名呈阳性。由于研究的患者数量有限，本研究是初步的。然而，这些结果表明 DST 与 CyA 组合仍然可以诱导抑制细胞，同时最大限度地减少致敏。

项目成果

Masaru Murai: "Effect of DST combined with cyclosporine on lymphocyte subsets of potential recipients for living related donor allograft." Transplantation Proceedings. 26. 1950-1951 (1995)

Masaru Murai：“DST 联合环孢素对活体相关供体同种异体移植潜在受体淋巴细胞亚群的影响。”

Masaru Murai: "Effect of DST combined with cycloporine on lymphocyte subsets of potential recipients for living related donor allograft." Geriatric Medicine. 26. 1950-1951 (1996)

Masaru Murai：“DST 联合环孢素对活体相关供体同种异体移植潜在受体淋巴细胞亚群的影响。”

M.Murai, H.Nakamura, S.Sekiguchi, S.Shimizu and F.Nakajima: "Effect of DST combined with cyclosporine on lymphocyte subsets of potential recipients for living-related donor renal allograft." Transplantation Proceedings. 26. 1950-1951 (1995)

M.Murai、H.Nakamura、S.Sekiguchi、S.Shimizu 和 F.Nakajima：“DST 联合环孢素对活体相关供体肾同种异体移植潜在受体淋巴细胞亚群的影响”。

村井 勝: "高尿酸血症(腎移植患者の管理)" 腎と透析. 41(Supple). 675-676 (1996)

Masaru Murai：“高尿酸血症（肾移植患者的管理）”《肾脏与透析》41（补充）675-676（1996）。

村井 勝: "系統看護学講座" 医学書院, 275 (1995)

村井胜：《系统护理课程》医学书院，275（1995）