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PRESENCE AND POSSIBLE FUNCTION OF GROWTH HORMONE BINDING PROTEIN IN HUMAN FOLLICULAR FLUID

人类卵泡液中生长激素结合蛋白的存在及其可能的功能

基本信息

批准号：
07671820
负责人：
KINOSHITA Katuyuki
金额：
$ 1.41万
依托单位：
SAITAMA MEDICAL SCHOOL
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671820/
关键词：
GROWTH HORMONE GROWTH HORMONE BINDING PROTEIN FOLLICULAR FLUID IN VITRO FERTILIZATION FOLLICULOGENESIS OVARIAN RESERVE 体外受精胚移植 IGF-1 卵胞液

项目摘要

In order to elucidate the function of growth hormone (GH) in ovarian folliculogenesis, we studied presence and possible function of growth hormone binding protein (GHBP) in sera and follicular fluids of patients undergoing in vitro fertilization. The main results are as follows. 1. GHBP in the levels of 3-32ng/ml existed in the sera and follicular fluids at the time of oocyte pickup. 2. There was no difference in the levels of GHBP among follicles in each patient. The serum levels of GHBP equaled to those of follicles. 3. The levels of GHBP in the sera were relatively stable over the course of ovarian hyperstimulation despite the rising levels of serum estradiol. 4. The numbers of oocytes obtained in the patients with higher serum levels of GHBP (>20ng/ml) were significantly (p<0.05) lower than those of the patients with lower serum levels of GHBP (<20ng/ml). 5.By RT-PCR we detected growth hormone receptor mRNA in the granulosa cells obtained at the time of oocyte pickup. However, there was no correlation between the serum GHBP levels and the growth hormone receptor expression in the granulosa cells in the patients studied. In conclusion, GHBP may have a role in ovarian folliculogenesis by modulating GH action in sera and follicular fluid. The serum GHBP levels may serve as a possible marker of ovarian reserve in the patients undergoing ovarian hyperstimulation.

为了阐明生长激素（GH）在卵泡发生中的作用，我们研究了体外受精患者血清和卵泡液中生长激素结合蛋白（GHBP）的存在及其可能的功能。主要结果如下。1.取卵时血清和卵泡液中GHBP含量为3- 32 ng/ml。2.每个患者卵泡中GHBP水平无差异。血清GHBP水平与卵泡水平相当。3.在卵巢过度刺激过程中，尽管血清雌二醇水平升高，但血清GHBP水平相对稳定。4.血清GHBP水平高者（> 20 ng/ml）获卵数显著低于血清GHBP水平低者（<20 ng/ml）（p<0.05）。5.采用RT-PCR方法检测取卵时颗粒细胞中生长激素受体mRNA的表达。然而，在研究的患者中，血清GHBP水平与颗粒细胞中生长激素受体表达之间没有相关性。总之，GHBP可能通过调节血清和卵泡液中GH的作用而在卵泡发生中发挥作用。血清GHBP水平可作为超排卵患者卵巢储备功能的一个可能的指标。