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Oncogene products associated with apoptosis and cell proliferation during carcinogenesis of uterine cervical squamous epithelium.

宫颈鳞状上皮癌变过程中与细胞凋亡和细胞增殖相关的癌基因产物。

基本信息

批准号：
07671834
负责人：
YABUSHITA Hiromitsu
金额：
$ 1.47万
依托单位：
AICHI MEDICAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07671834/
关键词：
cervical cancer apoptosis cell proliferation CIN HPV oncogene product 子宮頚癌子宮頚部異形成増殖相細胞 p53 bcl-2

项目摘要

To assess the biological characteristics of cervical intraepithelial neoplasia (CIN) with high-risk HPV infection, both the apoptotic cell population (%Apo) and the proliferating cell population (%GF) were examined using a formalin-fixed paraffinembedded section obtained from 38 patients with CIN,all of whom HPV 18/16 E6 was detected immunohistochemically. The relations of %Apo or %GF to an immunohistochemical expression of p53, bcl-2, Rb, Bax and WAF1 were also investigated here. The %Apo was significantly lower in the CIN III group than the CIN I,II group, but the %GF was significantly higher in the former than the latter. The frequency of expression for p53 or WAF1 was increased in CIN III group compared to CIN I,II group, and that for Bax was decreased in the former compared to the latter, but that for bcl-2 or Rb was not different between CIN I,II group and CIN III group. The %Apo decreased in both CIN III cases and CIN I,II cases when expressing a bcl-2, and it increased in CIN III cases expressing Bax. In contrast, the %GF elevated in CIN I,II cases expressing p53 or Rb, and in CIN III cases expressing WAF1. Above result concluded us that a decrease in apoptosis during CIN was induced by the regress in Bax expression, and that an increase in cell proliferative activity was induced by the progress in the expression for p53, Rb and/or WAF1, although no molecular biological study was conducted for the specimens used for this study.

为探讨宫颈上皮内瘤变(CIN)高危HPV感染的生物学特征，采用免疫组织化学方法检测38例CIN患者宫颈上皮内瘤变(CIN)组织中HPV18/16E6的凋亡细胞数(%Apo)和增殖细胞数(%GF)。本文还探讨了%Apo或%GF与P53、BCL-2、Rb、Bax和WAF1免疫组织化学表达的关系。CIN III组的%Apo显著低于CIN I、II组，而%GF显著高于CIN I、II组。P53、WAF1在CIN III组中的表达频率高于CIN I、II组，bax的表达频率在CIN I、II组中低于CIN III组，而bcl2、Rb的表达频率在CIN I、II组与CIN III组之间无差异。在表达bcl2的CIN III和CIN I、II中，Apo值均降低，而表达bax的CIN III中Apo值升高。而在表达P53或Rb的CIN I、CIN II和表达WAF1的CIN III中，%GF均升高。上述结果提示，尽管未对本研究中使用的标本进行分子生物学研究，但在CIN过程中，Bax表达的减退导致了细胞凋亡的减少，而P53、Rb和/或WAF1的表达则导致了细胞增殖活性的增强。