Pharmacological studies on the effects of kanpo medicine on the injury of rat liver by ischemia-reperfusion.

对10周龄雄性Wistar大鼠口服100 mg/kg剂量的“Shosaikoto”4天，并在最后一次给药后1小时通过1小时肝缺血制备实验性肝损伤模型。肝脏再灌注1小时后，测定血清中谷草转氨酶（GOT）、谷丙转氨酶（GPT）和乳酸脱氢酶（LDH）。测量肝匀浆中的脂质过氧化物，并表示为每mg蛋白质形成的丙二醛的nmol。测定了总谷胱甘肽含量、细胞色素P-450、细胞色素b_5和NADPH细胞色素C还原酶活性。对照组大鼠脂质过氧化物含量为6.82 ± <plus-minus>0.54，服用“Shosaikoto”组大鼠脂质过氧化物含量为5.11 ± <plus-minus>0.15。而总谷胱甘肽含量、细胞色素P-450、细胞色素b_5和NADPH细胞色素C还原酶活性则无明显变化。“Shosaikoto”似乎不能防止肝损伤。实验结束后，分别以100、200、400 mg/kg剂量组和100 mg/kg剂量组 关于我们 8周龄雄性Wistar大鼠经口给予200和400 mg/kg的苯妥英钠，连续4天，末次给药后1小时，通过15分钟肝缺血制备中度实验性肝损伤模型。肝脏再灌注1小时后，测定血清中谷草转氨酶（GOT）、谷丙转氨酶（GPT）和乳酸脱氢酶（LDH）。测量肝匀浆中的脂质过氧化物，并表示为每mg蛋白质形成的丙二醛的nmol。脂质过氧化物的含量对照组（缺血）为7.06 ± <plus-minus>0.67和200 mg/kg的“大柴胡”治疗组分别为6.58 <plus-minus>± 0.33和6.33 ± <plus-minus>0.55。因此，“大柴胡汤”倾向于减少含量。然而，200 mg/kg和400 mg/kg“Hochuekkito”处理的大鼠的含量没有降低。通过“Daisaikoto”和“Hochuekito”治疗观察到肝损伤轻微缓解。可以得出结论，缓解肝损伤的“Shosaikoto”和“Daisaikoto”可能会发生捕获脂质过氧化物。这可能是通过不同的机制发生的。少

"Shosaikoto" in a dose of 100 mg/kg was orally administered for 4 days to 10 week old male Wistar rats and the experimental liver injury model was prepared by 1 hr liver ischemia 1 hr after last administration. After 1 hr blood reperfusion of the liver, GOT,GPT and LDH in the serum were estimated. Lipid peroxides in liver homogenates were measured and expressed as nmol of malonic dialdehyde formed per mg protein. Content of total glutathion, activities of cytochrome P-450, cytochrome b_5 and NADPH cytochrome Creductase were also estimated. The contents of lipid peroxide of control rats were 6.82 <plus-minus>0.54 and those of rats treated with "Shosaikoto" was decreased to 5.11<plus-minus>0.15. However, contents of total glutathion, activities of cytochrome P-450, cytochrome b_5 and NADPH cytochrome C reductase were not altered. "Shosaikoto" dose not seem to protect against liver injury. Following above experiments, "Daisaikoto" in doses of 100,200 and 400 mg/kg, or "Hochuekkito" in dos … More es of 200 and 400 mg/kg were orally administrered for 4 days to 8 week old male Wistar rats and the moderate experimental liver injury model was prepared by 15 min liver ischemia 1 hr after last administration. After 1 hr blood reperfusion of the liver, GOT,GPT and LDH in the serum were estimated. Lipid peroxides in liver homogenates were measured and expressed as n mol of malonic dialdehyde formed per mg protein. Contents of lipid peroxides of control rats (ischemia) was 7.06 <plus-minus>0.67 and those of rats treated with 200 mg/kg of "Daisaikoto" were 6.58<plus-minus>0.33 and 6.33 <plus-minus>0.55, respectively. Therefore, "Daisaikoto" tended to decrease the contents. However, the contents of rats treated with 200 mg/kg and 400 mg/kg of "Hochuekkito" were not decreased. Slight remission of liver injury was observed by treatment with "Daisaikoto" and "Hochuekito". It is concluded that remission of liver injury by "Shosaikoto" and "Daisaikoto" may occur by trapping lipid peroxides. That by "Hochuekito" may occur by different mechanism. Less