Random Amplified Polymorphic DNA (RAPD) Analyzes for Discriminating Genotypes of Microcystis Cyanobactcria and Its Application for the Management of Water Quality of Lakes

应用随机扩增多态性DNA(RAPD)技术对5种蓝藻的核型进行了分析。具有相同等位酶基因的每一组菌株具有各自所属组的相似的随机扩增多态性DNA图谱。另一方面，不同等位酶基因分型的菌株之间没有观察到任何相似之处。结果表明，两种方法对微囊藻亲缘类群的鉴别具有较高的可靠性。几个扩增的DNA片段也被观察到，这些片段有望作为具有相同等位酶基因的特定分类群的标记。通过随机扩增多态性DNA分析和等位酶基因分型，证明了诺瓦克分枝杆菌、绿色分枝杆菌和韦森伯格氏分枝杆菌的遗传同源性。然而，铜绿假单胞菌和成鱼分枝杆菌在DNA和蛋白质(等位酶)水平上存在显著差异。

Random amplified polymorphic DNA (RAPD) analysis was applied to the discrimination of gcnotypes in five species of Microcystis cyanobacteria. Strains of each group possessing the identical allozyme genotype gave similar RAPD patterns characterized by the respective group. On the other hand, any similarities in RAPD patterns were not observed among strains of which allozyme genotypes were different. A good accordance between the RAPD analysis and allozyme divergence indicated a high reliability of the both methods for discrimination of the affiliated group of Microcystis. Several amplified DNA fragments, which were expected as markers for a particular taxon with identical allozyme genotype, were also observed on the RAPD patterns. Genetic homogenieties of M.novacekii, M.viridis and M.wesenbergii were shown by RAPD analysis as well as allozyme genotypes. However, significant variations were observed in M.aeruginosa and M.ichthyoblabe in the levels of DNA and proteins (allozymes).