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Molecular evolution of Galectins and their ligands

半乳糖凝集素及其配体的分子进化

基本信息

批准号：
07680659
负责人：
KASAI Ken-ichi
金额：
$ 1.28万
依托单位：
Teikyo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680659/
关键词：
Galectin Neatode C.elegans Glycoconjugate Lectin ガクレチン皮膚

项目摘要

The complete structure of the gene for 32-kDa galection of the nematode Caenorhabditis elegans, which is the firstly found invertebrate galectin, was determined. Only two introns were found. The insertion position of one intron corresponds to those found in the cases of vertebrate galectins, while that of the other was unique. We also found another galectin from C.elegans, which corresponds to the proto type of vertebrates, cloned its cDNA,and determined its whole structure. The finding that both vertebrates and C.elegans have a similar set of galectins strongly suggests the existence of common biological roles that shuould be indespensable for multicellular animals. Since many galectin-like sequences (abou 10) have been found in the genome of C.elegans as a result of the genome project, these sequences were cloned from a cDNA library into an expression vector in order to confirm whether they are functional genes or not. Some sequences were shown to actually encode proteins having sugar-binding ability. It is interesting that C.elegans has as many galectins as mammals do. Glycoproteins as possible candidates for endogenous ligands for galectins have been isolated, and their structural features are now being investigated. Localization of galectins investigated by immunohistochemical technique also indicated the existence of common biological functions between C.elegans and mammals. An efficient analytical procedure based on capillary electrophoresis for comparison of functions of galectins of different origins was also developed in this research project.

测定了首次发现的无脊椎动物秀丽线虫的32 kDa Galectin基因的完整结构。只发现了两个内含子。一个内含子的插入位置与脊椎动物Galectins中发现的位置相对应，而另一个内含子的插入位置是独特的。我们还从线虫中发现了另一种对应于脊椎动物原始型的Galectin，克隆了它的cDNA，并确定了它的整体结构。脊椎动物和线虫都有一组相似的Galectins，这一发现有力地表明，存在着多细胞动物不可忽视的共同生物学作用。由于基因组计划在线虫基因组中发现了许多类似半乳糖凝集素的序列(Abou 10)，因此将这些序列从cDNA文库克隆到表达载体中，以确定它们是否是功能基因。一些序列实际上编码了具有糖结合能力的蛋白质。有趣的是，线虫的Galectin和哺乳动物一样多。作为半乳糖凝集素内源性配体的候选糖蛋白已经被分离出来，其结构特征正在研究中。用免疫组织化学技术研究Galectins的定位也表明线虫和哺乳动物之间存在共同的生物学功能。本研究还开发了一种基于毛细管电泳法的高效分析方法，用于比较不同来源的Galectins的功能。