Analysis of the tax-4 gene encoding a cyclic nucleotidegated channel in C.elegans

线虫中编码环核苷酸门控通道的tax-4基因的分析

• 批准号: 07680724
• 负责人: MORI Ikue
• 金额: $ 0.38万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07680724/
• 关键词: C.elegans; thermotaxis; chemotaxis; molecular genetics; cation channel; C. elegans

The C.elegans tax-4 mutants are abnormal in multiple sensory behaviors : they fail to respond to temperature, watersoluble or volatile chemical attractants. We show that the predicted tax-4 gene product is highly homologous to vertebrate cyclic nucleotide-gated channels. Tax-4 protein expressed in cultured cells functions as a cyclic nucleotide-gated channel. The GFP-tagged functional Tax-4 protein is expressed in thermosensory, gustatory and olfactory neurons mediating all the sensory behaviors affected by the tax-4 mutations. The Tax-4/GFP fusion is partly localized at the sensory endings of these neurons. The results suggest that a cyclic nucleotide-gated channel is required for thermosensation and chemosensation, and that cGMP is an important intracellular messenger in C.elegans sensory transduction.

秀丽隐杆线虫tax-4突变体在多个感觉行为中是异常的：它们不能对温度、水溶性或挥发性化学引诱剂作出反应。我们表明，预测的税收-4基因产物是高度同源的脊椎动物环核苷酸门控通道。在培养细胞中表达的Tax-4蛋白作为环核苷酸门控通道发挥功能。GFP标记的功能性Tax-4蛋白在热敏、味觉和嗅觉神经元中表达，介导受Tax-4突变影响的所有感觉行为。Tax-4/GFP融合部分位于这些神经元的感觉末梢。结果表明，环核苷酸门控通道是必需的温度和化学感觉，cGMP是一个重要的细胞内信使在秀丽隐杆线虫的感觉转导。

项目成果

Komatsu, H: "Mutations in a cyclic nucleotide-gated channel lead to abnormal thermosensation and chemosensation in C.elegans" Neuron. 17. 707-718 (1996)

Komatsu, H：“环核苷酸门控通道中的突变导致线虫中异常的热感觉和化学感觉”神经元。