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Establishment of an animal model for mammary cancer metastasis, and the search for anti-metastatic substances

乳腺癌转移动物模型的建立及抗转移物质的寻找

基本信息

批准号：
07680924
负责人：
MORIMOTO Junji
金额：
$ 1.47万
依托单位：
Osaka Medical College
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1997
项目状态：
已结题

项目摘要

We have been studying cancer metastasis (animal model for cancer metastasis and anti-metastatic substances), and obtained following results with support by the grant-in-aid for scientific research.1. Using mouse and human breast cancer cell lines, we compared the susceptibility to metastasis among the immunodeficient mouse strains BALB/c-nude, Lasat and SCID, and found SCID mice to have advantages over BALB/c-nude and Lasat mice. in frequency of incidence of metastasis and condition of metastatic colonies.2. We newly established a high mammary cancer strain of mice with severe pulmonary metastasis by infecting BALB/c mice with JygMMTV.With repeated sib-mating, we have reached the F9 to F10 generations at present. At the F6 generation, the incidence of cancer is 91%, the average age of incidence is 11.2 months. and the metastatic rate is 72% (microscopically).3. With repeated subcutaneous transplantation of lung metastatic tissues, we produced an animal model for cancer metastasis. In this model, we can visually observe almost 100% lung metastasis within 2-3 months after, transplantation. We are also currently trying to establish a lymphatic metastasis model using the same method.4. We established a new munne mammary cancer cell line (BJMC338) from the mammary tumor of a JygMMTV infected BALB/c mouse, with metastatic colonies in the lung and liver.This cell line is useful as an experimental metastatic model with an i.v. of cells, and not so with s.c., spontaneous metastatic research.5. Next we searched for substances with anti-cancer and metastatic activities in marine biomatenals, especially sea weeds. Using the mouse melanoma cell (B16-BL6) and mammary cancer cell (BJMC338) model we established, we found that Marginisporum crassissium, and Laminaria anguestata inhibit cancer metastasis in the melanoma model, and Laniinaria anguestata does so in the inanurlary cancer model.

本研究所在科学研究补助金的支持下，进行了癌转移（癌转移的动物模型和抗转移物质）的研究，取得了以下成果。1.使用小鼠和人乳腺癌细胞系，我们比较了免疫缺陷小鼠品系BALB/c-nude，Lasat和SCID之间的转移敏感性，发现SCID小鼠具有优于BALB/c-nude和Lasat小鼠的优势。与转移发生率及转移灶情况有关.我们用JygMMTV感染BALB/c小鼠，建立了一个高乳腺癌肺转移小鼠品系，经反复交配，目前已传至F9 ~ F10代。在F6代，癌症的发病率为91%，平均发病年龄为11.2个月。转移率为72%（镜下）.通过重复皮下移植肺转移组织，我们建立了癌症转移的动物模型。在该模型中，我们可以在移植后2-3个月内直观地观察到几乎100%的肺转移。我们目前也在尝试用同样的方法建立淋巴转移模型.我们从JygMMTV感染的BALB/c小鼠的乳腺肿瘤中建立了一个新的munne乳腺癌细胞系（BJMC 338），该细胞系在肺和肝中具有转移集落。该细胞系可用作i. v.细胞的实验转移模型，而不是s.c.，自发转移研究。接下来，我们在海洋生物材料，特别是海藻中寻找具有抗癌和转移活性的物质。利用我们建立的小鼠黑色素瘤细胞（B16-BL 6）和乳腺癌细胞（BJMC 338）模型，我们发现厚缘孢霉和狭叶海带在黑色素瘤模型中抑制癌症转移，而狭叶羊毛衫在非肿瘤模型中也有此作用。