Establishment of an animal model for mammary cancer metastasis, and the search for anti-metastatic substances

我们一直在研究肿瘤转移(肿瘤转移和抗转移物质的动物模型)，并在科学研究补助金的支持下取得了以下结果。利用小鼠和人乳腺癌细胞株，比较了免疫缺陷小鼠BALB/c-nude、Lasat和SCID对转移的敏感性，发现SCID小鼠比BALB/c-nude和Lasat小鼠更具优势。在转移发生率和转移结节情况方面。我们用JygMMTV感染BALB/c小鼠，建立了一株高度乳腺癌、严重肺转移的小鼠，经反复同胞交配，目前已传至F9~F10代。在F6代，癌症发病率为91%，平均发病年龄为11.2个月。转移率为72%(显微镜下)。通过反复皮下移植肺转移组织，我们建立了一种肿瘤转移的动物模型。在这个模型中，我们可以在移植后2-3个月内直观地观察到几乎100%的肺转移。我们目前也在尝试用同样的方法建立淋巴转移模型。我们从感染JygMMTV的BALB/c小鼠的乳腺肿瘤中建立了一种新的小鼠乳腺癌细胞系(BJMC338)，该细胞系在肺和肝脏有转移集落，可作为静脉注射建立实验性转移模型。细胞的自发性转移研究，而S.C.并非如此。接下来，我们在海洋生物，特别是海藻中寻找具有抗癌和转移活性的物质。利用我们建立的小鼠黑色素瘤细胞(B16-BL6)和乳腺癌细胞(BJMC338)模型，我们发现马钱霉和海带在黑色素瘤模型中具有抑制肿瘤转移的作用，而海带在神经癌模型中具有抑制肿瘤转移的作用。

We have been studying cancer metastasis (animal model for cancer metastasis and anti-metastatic substances), and obtained following results with support by the grant-in-aid for scientific research.1. Using mouse and human breast cancer cell lines, we compared the susceptibility to metastasis among the immunodeficient mouse strains BALB/c-nude, Lasat and SCID, and found SCID mice to have advantages over BALB/c-nude and Lasat mice. in frequency of incidence of metastasis and condition of metastatic colonies.2. We newly established a high mammary cancer strain of mice with severe pulmonary metastasis by infecting BALB/c mice with JygMMTV.With repeated sib-mating, we have reached the F9 to F10 generations at present. At the F6 generation, the incidence of cancer is 91%, the average age of incidence is 11.2 months. and the metastatic rate is 72% (microscopically).3. With repeated subcutaneous transplantation of lung metastatic tissues, we produced an animal model for cancer metastasis. In this model, we can visually observe almost 100% lung metastasis within 2-3 months after, transplantation. We are also currently trying to establish a lymphatic metastasis model using the same method.4. We established a new munne mammary cancer cell line (BJMC338) from the mammary tumor of a JygMMTV infected BALB/c mouse, with metastatic colonies in the lung and liver.This cell line is useful as an experimental metastatic model with an i.v. of cells, and not so with s.c., spontaneous metastatic research.5. Next we searched for substances with anti-cancer and metastatic activities in marine biomatenals, especially sea weeds. Using the mouse melanoma cell (B16-BL6) and mammary cancer cell (BJMC338) model we established, we found that Marginisporum crassissium, and Laminaria anguestata inhibit cancer metastasis in the melanoma model, and Laniinaria anguestata does so in the inanurlary cancer model.