肝類洞内皮細胞による類洞微小循環調節機序

肝窦内皮细胞对肝窦微循环的调节

• 批准号: 07770407
• 负责人: 釈迦堂 敏
• 金额: $ 0.51万
• 依托单位: Kurume University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07770407/
• 关键词: 肝類洞内皮細胞; 培養; マトリゲル; 血管新生

ウイスター系雄性ラットより、コラゲナーゼ消化法及びelutriation rotorを用いて、肝類洞内皮細胞の分離を行った。分離細胞はマトリゲル上で培養した。培養3時間後より、ビデオで経時的に観察した。マトリゲル上の培養内皮細胞は、6時間後より遊走を始め、12時間後には管腔を形成した。管腔形成した後に、血管作動性物質を培養液中に加え、管腔の収縮、弛緩をビデオで観察した。アセチルコリン、サブスタンスP、エンドセリン、プロスタグランディンを加えて経時的に観察したが、ビデオでの観察では、管腔の収縮、弛緩は観察されなかった。今後は電顕的な観察によって、管腔の収縮、弛緩を定量化する必要がある。血管新生因子が類洞内皮細胞に及ぼす影響を観察するため、EGF (epidermal growth gactor)、acidic FGF (fibroglast growth factor)、basic FGF (fibroglast growth factor)、HGF (hepatocytes growth factor)、VEGF (vescular endothelial cell growth factor)を培養肝類洞内皮細胞に添加した。EGF、acidic FGFは培養肝類洞内皮細胞に影響は及ぼさなかったが、basic FGF、HGFは内皮細胞の管腔形成は抑制した。いずれの増殖因子を用いても、類洞内皮細胞に特異的な内皮細胞小孔は保持されていた。また、Factor VIIIも染色されず、類洞内皮細胞の機能は保持されていると考えられた。肝癌増殖時や肝再生時には、これらの増殖因子が作用して類洞内皮細胞の増殖と類洞の再生が起こるものと思われた。

The methods of digestion, elutriation rotor and hepatoendotheliocyte isolation were used in the male and hepatic cavities. Separate the cells from each other and make sure that you don't know what to do. After 3 hours of training, during the morning and morning hours, the training will be carried out in the morning. The endothelial cells were cultured at 6: 00, and the lumen was formed at 12: 00. After the formation of the lumen, the vascular action substance was added to the solution, the lumen was added, and the lumen was relaxed. Make sure that you don't know what's going on when you're in the middle of an emergency, that's why you're going to do so, and that's why you're not going to be able to do so. In the future, it is necessary to make a quantitative analysis of the electrical equipment, the lumen, and the relaxation of the electric power station. Angiogenesis factors (Angiogenesis Factor), EGF (epidermal growth gactor), acidic FGF (fibroglast growth factor), basic FGF (fibroglast growth factor), HGF (hepatocytes growth factor), VEGF (vescular endothelial cell growth factor) were used to detect the addition of hepatocyte endothelial cells. EGF and acidic FGF were used to culture the endothelial cell shadow and the endothelial cell lumen of EGF, basic FGF and HGF to form inhibitory lumen. The micropores of endothelial cells, which are specific to the endothelial cells of the same kind of holes, are kept in place by using the micropore of the endothelial cell of the same kind of hole. The endothelial cell scavenging machine of Factor and staining can be used to maintain the accuracy of the test. The role of cytokines in liver regeneration during liver regeneration plays an important role in the proliferation of hepatocellular carcinoma (HCC).

项目成果

Satoshi SHAKADO: "Effects of extracellular matrices on tube formation of cultured rat hepatic sinusoidal endothelial cells" Hepatology. 22. 969-973 (1995)

Satoshi SHAKADO：“细胞外基质对培养的大鼠肝窦内皮细胞管形成的影响”肝病学。