胎児発育と成長因子IGF familyの動態とその生理学的意義

胎儿发育和生长因子IGF家族的动态及其生理意义

• 批准号: 07770919
• 负责人: 船越 徹
• 金额: $ 0.51万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07770919/
• 关键词: IGF-I; IGFBP; IGFレセプター; GH; GHレセプター; 胎児発育; 妊娠; 異常妊娠

1.臨床的検討インフォームドコンセントが得られた正常妊婦,糖代謝異常合併妊婦,肥満妊婦,妊娠中毒症,重症妊娠悪阻妊婦,早産妊婦,その他各種合併症を持つ妊婦,また喫煙妊婦,および超音波断層法にて子宮内胎児発育遅延や巨大児と推定された妊婦より血液を,またこれら妊婦の分娩時に新生児臍帯血を採取し、血中総IGF-I測定を行った。結果,母体血では妊娠中毒症,重症妊娠悪阻妊婦では総IGF-Iは有意に低かった。新生児臍帯血では児の出生時体重と有意な相関を示した。今後、同サンプルのIGFBP分析を行う。また,得られた結果を妊娠週数,採血時の母体体重,母体体格Body Mass Index(BMI),採血時の母体体重増加(妊娠母体体重・非妊時体重),合併症別,その病勢の経過,新生児の出生時体重,児の体格等で分析する。基礎的検討ラットを用いて交配させて胎仔期より新生仔期,成体までの血清および肝臓のサンプリングを行った。今後,血中GH,肝のGHレセプター,IGF-Iの血中濃度,肝のIGFレセプターおよびIGFBPを分析し,それらの相互の関連を検討する。IGFBPの分析はゲル濾過法,Western ligand bloting法,ligand cross-linking法で行う。またヒトの血中BP1とBP3濃度はEnzyme-linked immunosorbent assayにて測定する。

1. Normal pregnancy, hyperglycemia, toxicosis, severe pregnancy, early pregnancy, pregnancy, During the delivery of pregnancy, the blood samples were collected and the IGF-I in the blood was determined. The results showed that the maternal blood had toxicosis in pregnancy, and the IGF-I in severe pregnancy was intentionally low. The birth weight of the newborns was intentionally related to each other. From now on, we will do the same for IGFBP analysis. The results of pregnancy, maternal weight at birth, maternal physical Body Mass Index (BMI), maternal weight at pregnancy (maternal weight during pregnancy and non-pregnancy weight), clinical symptoms, maternal weight at birth, birth weight at birth and maternal physique at birth were analyzed. She used mating, pregnancy, newborn, adult, serum, liver, and so on. From now on, GH in the blood, GH in the liver, IGF-I in the blood, IGF in the liver, IGFBP analysis in the liver, each other in each other. IGFBP analysis method, Western ligand bloting method and ligand cross-linking method were used to analyze the results. The BP1 in the blood was measured by BP3 and Enzyme-linked immunosorbent assay.