op/opマウスを用いたマクロファージの卵胞発育および排卵に及ぼす影響の検討

使用 op/op 小鼠检查巨噬细胞对卵泡发育和排卵的影响

• 批准号: 07771387
• 负责人: 福松 之敦
• 金额: $ 0.7万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07771387/
• 关键词: 卵胞発育; マクロファージ; op; opマウス; M-CSF

卵胞発育および排卵に対するマクロファージとM-CSFの生体内での関与を知る目的で、M-CSFが欠損し各臓器のマクロファージ数が著減しているop/opマウスを用いて検討を行った。op/opマウスとそのヘテロ接合体、さらに生後10日目よりhuman recombinant M-CSF 5μgを連日皮下投与したop/opマウスを、午前7時点灯午後7時消灯の条件下に16〜18週齢まで飼育した。腟スメアにて自然性周期を判定し、発情前期午後7時に卵巣を採取し、その連続切片にて発育および成熟卵胞数を計測した。連続切片の一部は抗マウスマクロファージモノクローナル抗体F4/80を用いて免疫組織化学的染色を行い、最大径が300μmを示す発育卵胞の切片における顆粒膜細胞およびマクロファージ数を計測した。また、発情前期午後5時に[^3H]thymidine 55kBq/gを腹腔内投与し、2時間後に卵巣を採取し、顆粒膜細胞の増殖能を検討した。さらに、発情後期午前11時に両側卵管に排出された卵の数を計測した。その結果、発育卵胞内マクロファージ数は、op/opマウスでそのヘテロ接合体に比して有意に少なく、M-CSFの投与で増加した。発育卵胞数および成熟卵胞数も同様に、op/opマウスではそのヘテロ接合体に比して有意に少なく、M-CSFの投与で増加した。発育卵胞の顆粒膜細胞の増殖能もop/opマウスで低く、M-CSFの投与で増加した。排卵個数もop/opマウスではヘテロ接合体に比して有意に少なく、M-CSFの投与で増加した。以上、M-CSFあるいはマクロファージが卵胞発育と排卵に促進的に作用していることが示された。

Ovulation, ovulation After 10 days of birth, the joint was subcutaneously injected into the op/op after 10 days of birth, the light was lit at 7: 00 in the morning and the light was turned off at 7: 00 in the afternoon. Under the condition of 7: 00 in the afternoon and 7: 00 in the afternoon, it was 16: 18 at the age of 18. The natural sexual cycle was determined, the oocytes were collected at 7: 00 p. M. in the proestrus, and the number of mature oocytes was calculated. A linked section of the oocyte was stained with immunohisto-chemical staining and the maximum diameter was 300 μ m. The largest diameter of the section was 300 μ m. The maximum diameter was 300 μ m. Thymidine 55kBq/g was injected intraperitoneally at 5: 00 p. M. in proestrus and proestrus. After 2: 00 p. M., ovum was harvested and granulocyte membrane colonization was performed. The number of eggs excreted from the oviduct was calculated at 11: 00 p. M. in post-estrus. The results of the experiment, the number of embryos in the oocyte, the number of conjugates in the op/op cells, the number of conjugates in the oocytes, the number of conjugates in the oocytes, and the number of conjugates in the oocytes were less than those in the oocytes, and the number of conjugates in the oocytes was less than that in the oocytes, and the number of conjugates in the oocytes was less than that in the oocytes, and the number of conjugates was less than that in the oocytes. The number of eggs, the number of mature eggs, the number of mature eggs, the number of The fecundity of the oocyte, the membrane cell, the op/op cell, the low egg cell, the M-CSF cell, the egg membrane cell, the egg cell, the egg cell, the The number of ovulations was less in op/op recipients than in pregnant women, and the number of M-CSF implants was less than that in pregnant women. Above, the effect of M-CSF on ovulation and ovulation has been shown in this article.

项目成果

Masako Araki: "Follicular Development and Ovuration in Macrophage Colony-Stimulating Factor-Deficient Mice Homozygous for the Osteopetrosis (op) Mutation" BIOLOGY OF REPRODUCTION. 54. 478-484 (1996)

Masako Araki：“骨石症（op）突变纯合巨噬细胞集落刺激因子缺陷小鼠的卵泡发育和排卵”生殖生物学。