The role of cell adhesion molecules of inflammatory cell & brain endothe-lial cell in post brain ischemic/hypoxic hypoperfusion

再灌注/复氧后脑灌注不足在诸如心脏骤停、休克、脑外伤和脑梗塞的情况下是严重的问题。观察了多形核白细胞（PMNL）和脑内皮细胞粘附分子（CADM）对麻醉期间复氧后脑低灌注的影响。在体外实验中，我们研究了氯胺酮和硫喷妥钠对人未刺激的，fMLP和PMA刺激的PMNLs的细胞粘附分子表达的影响。本实验发现临床浓度的硫喷妥钠可抑制PMNLs上CD 11b的表达，但对CD 62 L的表达无明显影响，而吸入麻醉药对CADM的影响在体外实验中很难观察，因此，我们研究了异氟醚对CADM的影响（0.5 - 1.5MAC）对缺氧（5%O_2，10 min）-复氧（120 min）大鼠CADM表达的影响。脑灌注不足指标 ...更多信息 n，我们最初采用颅窗法测量血管直径。然而，这种方法由于打开颅骨而激活了PMNLs。相反，我们通过近红外光谱非侵入性地评估脑氧合。在0.5MAC异氟醚麻醉下，复氧后120 min总血红蛋白（tHb）和氧合血红蛋白（HbO_2）显著降低，提示复氧后出现低灌注。0.5MAC复氧后CD 11b升高，CD 11b与tHb、HbO_2呈显著负相关。相比之下，tHb、HbO_2和CD 11b的表达没有变化。酶免疫染色显示复氧后ICAM-1表达增加。然而，我们不能定量测量该CADM的表达。从这些研究结果中，我们建议，麻醉剂，如异氟烷和硫喷妥钠保护缺血或缺氧后的脑灌注不足，至少部分是由于衰减的PMNLs的细胞粘附分子的表达。少

Post reperfusion/reoxygenation brain hypoperfusion is a serious problem in conditions such as cardiac arrest, shock, brain trauma and cerebral infarction. We investigated the influence of cell adhesion molecules (CADM) of polymorphonuclear leukocyte (PMNL) and brain endothelial cells on post reoxygenation brain hypoperfusion during anesthesia. In vitro experiments, we investigated the effects of ketamine and thiopental on the expression of cell adhesion molecules of human unstimulated, fMLP- and PMA-stimulated PMNLs. From this experiment we found clinical concentration of thiopental, which has been reported to protect focal brain ischemia, attenuated the increase of CD11b on PMNLs, but not that of CD62L.In vitro experiment it is difficult to the effect of inhalation anesthetics on the CADM.Therefore, we investigated the influence of isoflurane (0.5 - 1.5 MAC) on the CADM expression after hypoxia (5% O_2, 10 min) -reoxygenation (120 mm) in rat. As to the indices of cerebral hypoperfusio … More n, we initially used cranial window method to measure the diameter of blood vessels. However, this method activated the PMNLs due to opening the cranium. Instead, we evaluated brain oxygenation by near infrared spectroscopy non-envasively. Under 0.5 MAC isoflurane anesthesia total Hb (tHb) and HbO_2 (oxy-hemoglobin) significantly decreased at 120 min after reoxygenation, suggesting that post-reoxygenation hypoperfusion occurred. The CD11b increased after reoxygenation at 0.5 MAC.There were significant negative relationshios between CD11b and tHb, and CD11b and HbO_2. In contrast, there was no change in tHb, HbO_2, and the expression of CD11b at. The 1.5 MAC.The ICAM-ldetected by enzyme immuno-staining increased after reoxygenation. However, we could not measure the expression of this CADM quantitatively. From these findings we suggested that anesthetics such as isoflurane and thiopental protected post ischemic or hypoxic brain hypoperfusion at least in part due to attenuation of the expression of cell adhesion molecules of PMNLs. Less