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VACUOLAR PROCESSING SYSTEM IN HIGHER PLANTS

高等植物中的液泡处理系统

基本信息

批准号：
08454262
负责人：
HARA Ikuko
金额：
$ 4.86万
依托单位：
(NATIONAL INSTITUTE FOR BASIC BIOLOGY) Okazaki National Research Institutes
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08454262/
关键词：
Vacuolar processing enzyme Senescence Cysteine proteinase Arabidopsis Vacuoles Stress 液胞抵抗性遺伝子葯 Circular cell cluster 傷害

项目摘要

Vacuolar processing enzymes (VPE) are cysteine proteinases responsible for maturation of various vacuolar proteins in plants. A larger precursor to VPE synthesized on rough endoplasmic reticulum is converted to an active enzyme in the vacuoles. We expressed a precursor to castor bean VPE in a pep4 strain of the yeast Saccharomyces cerevisiate to examine the mechanism of activation of VPE.Our findings suggest that an inactive VPE precursor synthesized on the endoplasmic reticulum is transported to the vacuoles in the yeast cells and then processed to make an active VPE by self-catalytic proteolysis within the vacuoles.Arabidopsis has three vacuolar processing enzymes (VPEs). The promoter of the betaVPE gene was found to direct the expression of the beta-glucuronidase reporter gene in the seeds, the pollen and the root tip of a transgenic Arabidopsis. On the other hand, the promoters of both the alphaVPE and gammaVPE genes were found to direct remarkably the eression in senescent tissue. Treatment with wounding, ethylene and salicylic acid induced the gene expression of the alphaVPE and gammaVPE genes, while jasmonate slightly induced that of the gammaVPE.Taken together, the results suggest that vegetative VPEs might regulate the activation of some functional proteins in the vegetative vacuoles.

液泡加工酶（VPE）是负责植物中各种液泡蛋白成熟的半胱氨酸蛋白酶。在粗面内质网上合成的较大的 VPE 前体在液泡中转化为活性酶。我们在酿酒酵母的 pep4 菌株中表达了蓖麻子 VPE 的前体，以研究 VPE 的激活机制。我们的研究结果表明，在内质网上合成的无活性 VPE 前体被转运到酵母细胞的液泡中，然后通过在酵母细胞内的自催化蛋白水解作用来加工产生活性 VPE。液泡。拟南芥具有三种液泡加工酶（VPE）。研究发现，βVPE 基因的启动子可指导转基因拟南芥种子、花粉和根尖中 β-葡萄糖醛酸酶报告基因的表达。另一方面，发现 alphaVPE 和 gammaVPE 基因的启动子显着指导衰老组织中的肿胀。创伤处理、乙烯和水杨酸诱导了 alphaVPE 和 gammaVPE 基因的表达，而茉莉酸轻微诱导了 gammaVPE 的基因表达。综上所述，结果表明营养性 VPE 可能调节营养性液泡中某些功能蛋白的激活。