Functionalization of Electrodes Using Monolayrs of Organic Thiolate Derivatives

1.去除吸附在Au电极上的有机硫醇化合物制备微电极组件。在KCN水溶液中浸泡涂有自组装硫代醇单分子膜的Au电极，导致化学吸附的硫酸盐分子的部分脱附。解吸是在硫酸盐涂层电极的针孔处开始的，解吸的孔均匀地分布在电极的整个表面。结果表明，该电极具有微电极组合的电化学活性特征。2.具有电化学活性取代基的有机硫醇单分子膜涂层的金电极的电化学性质由自组装…涂层金属电极的伏安曲线成功地推导出理论方程氧化还原活性硫代硫酸盐的更多混杂单层。得到的峰电流和峰电位的显式表达式在形式上与以前发表的使用Frumkin吸附等温式的表达式相似。3以戊二醛为交联剂，用有机硫代硫酸酯固定化电极表面的酶及其作为生物传感器的电化学性质，实现了胆固醇氧化酶、胆固醇酯酶和尿酸酶在单层2-氨基乙硫酸酯涂层的金电极上的固定化。研究发现，固定化胆固醇和胆固醇酯酶的电极对游离胆固醇和酯化胆固醇具有安培敏感性，而含有尿酸酶的电极对尿酸具有敏感性。研究了它们作为生物传感器的电化学性质。较少

In this study, investigations have been done concerning three subjects and significant results were obtained for each subject as given in the followings.1 Preparation of microelectrodes assembly by eliminating organic thiolates adsorbed on Au electrodeImmersion of an Au electrode coated with a self-assembled monolayr of alkanethiolate in KCN aqueous solution caused partial desorption of chemisorbed thiolate molecules. The desorption was initiated at pinholes of the thiolate-coated electrode, giving desorbed holes distributed homogeneously over th entire surface of the electrode. It was found that the resulting electrode exhibited electrochemical activities characteristics of a microelectrode assembly.2 Electrochemical properties of Au electrode coated with a monolayr of organic thiolates having electrochemically active substituentsTheoretical equations were successfully derived on current-voltage curves obtained by potential sweep measurements of metal electrode coated with a self-asse … More mbled monolayr of redox-active thiolates. Explicit expressions derived for the peak-current and the peak potential were similar in their forms to those published previously using the Frumkin adsorption isotherms. Furthermore, Au electrodes coated with monolayrs of thiolates having phenothiazine derivatives were prepared and their electrocatalytic oxidation of NADH were investigated in detail.3 Immobilization of enzymes on electrode surface with use organic thiolates and their electrochemical properties as biosensorsImmobilization of cholesterol oxidase, cholesterol esterase, and uricase on Au electrodes coated with a monolayr of 2-aminoethanethiolate have been achieved by using glutaraldehyde as a cross-linking agent. It was found that the electrode on which cholesterol and cholesterol esterase were immobilized posessed amperometric sensitivities to free cholesterol as well as esterified cholesterol, while the electrode having uricase exhibited sensitivities to uric acid. Their electrochemical properties as biosensors have been investigated. Less