Functionalization of Electrodes Using Monolayrs of Organic Thiolate Derivatives

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In this study, investigations have been done concerning three subjects and significant results were obtained for each subject as given in the followings.1 Preparation of microelectrodes assembly by eliminating organic thiolates adsorbed on Au electrodeImmersion of an Au electrode coated with a self-assembled monolayr of alkanethiolate in KCN aqueous solution caused partial desorption of chemisorbed thiolate molecules. The desorption was initiated at pinholes of the thiolate-coated electrode, giving desorbed holes distributed homogeneously over th entire surface of the electrode. It was found that the resulting electrode exhibited electrochemical activities characteristics of a microelectrode assembly.2 Electrochemical properties of Au electrode coated with a monolayr of organic thiolates having electrochemically active substituentsTheoretical equations were successfully derived on current-voltage curves obtained by potential sweep measurements of metal electrode coated with a self-asse … More mbled monolayr of redox-active thiolates. Explicit expressions derived for the peak-current and the peak potential were similar in their forms to those published previously using the Frumkin adsorption isotherms. Furthermore, Au electrodes coated with monolayrs of thiolates having phenothiazine derivatives were prepared and their electrocatalytic oxidation of NADH were investigated in detail.3 Immobilization of enzymes on electrode surface with use organic thiolates and their electrochemical properties as biosensorsImmobilization of cholesterol oxidase, cholesterol esterase, and uricase on Au electrodes coated with a monolayr of 2-aminoethanethiolate have been achieved by using glutaraldehyde as a cross-linking agent. It was found that the electrode on which cholesterol and cholesterol esterase were immobilized posessed amperometric sensitivities to free cholesterol as well as esterified cholesterol, while the electrode having uricase exhibited sensitivities to uric acid. Their electrochemical properties as biosensors have been investigated. Less